Original article

Abirami Lakshmy Jayachandran^1*,Ravinder Thyagarajan², Radhika Katragadda², Leela K.V³,Suganthi M⁴, Lavanya K⁴, Hemalatha S⁴

¹ Assistant Professor, Department of Microbiology, Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Madhurantakam Taluk, Kanchipuram,Tamil Nadu, India
² Professor, Department of Microbiology, Government Kilpauk Medical College Hospital, Chennai, Tamil Nadu, India
³ Assistant Professor, Department of Microbiology, Government Kilpauk Medical College Hospital, Chennai, Tamil Nadu, India
4 Associate Professor, Department of Microbiology, Government Kilpauk MedicalCollege Hospital, Chennai,Tamil Nadu, India

The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu

Address reprint requests to
*Dr. Abirami Lakshmy J, Number 36: D Block, Viduthalai Nagar, Mudaliarpet, Puducherry, 605004, India

Article citation: Abirami Lakshmy J, Thyagarajan R, Radhika K, Leela KV, Suganthi M, Lavanya K, Hemalatha S. Biofilm formation and methicillin resistance among the Staphylococcus aureus causing burn wound infections in a tertiary care hospital: a comparative study of the antibiotic susceptibility pattern between biofilm producers and non biofilm producers. J Pharm Biomed Sci 2016;06(03):179–183.
Available at www.jpbms.info

ABSTRACT
Background Staphylococcus aureus is one of the common bacteria implicated in burn wound infections that possess the ability to form biofilms.
Aim The present study aims to isolate S. aureus to identify the Methicillin resistant Staphylococcal isolates, to compare the antibiotic susceptibility pattern between biofilm and non biofilm producers. It also aims to identify the biofilm producers by Microtitre plate method.
Settings and Design Observational study.
Materials and Methods A total of 58 S. aureus were isolated from burn wound infections. Antibiotic susceptibility testing was done by Kirby–Bauer disc diffusion method. Methicillin resistance was identified by cefoxitin disc method. All the isolates were screened for biofilm production by microtitre plate method. Statistical analysis: Fisher exact and Chi square tests. p < 0.005 is considered significant.
Results Out of the 58 isolates, 26 (44.8%) were identified as MRSA. Biofilm production was detected in 31 (53.4%) of the isolates. Good sensitivity for gentamicin 41 (70%), amikacin 42 (72.4%), cefotaxime 42 (72%) and erythromycin 37 (63.7%). There was no significant difference in the antibiotic susceptibility pattern between biofilm and non biofilm producers.
Conclusion All burn wounds should be screened for the presence of bacteria with biofilm forming ability along with the detection of drug resistance. This will support in the early identification and help in choosing the appropriate antibiotic.

KEYWORDS biofilm formation, burn wounds, Staphylococcus aureus


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