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Name | Comparative Analysis of Various Physico-Chemical Properties of Lectins from Phaseolus Vulgaris Landraces of Uttarakhand |
Description | Original research article:- Rattan Deep Singh1, Juhi Mishra1, Anoop Kumar Dobriyal 2, *Aradhana1 1.Dept. of Biotechnology, S. Bhagwan Singh PG Institute of Biomedical Sciences and Research, Balawala, Dehradun, India. 2.Dept. of Zoology and Biotechnology, Hemwati Nandan Bahuguna Garhwal University, Pauri Campus, Pauri-Garhwal, India.
Abstract:- The present study is based on analysis of various physio-chemical properties of crude extract of lectins from selected Phaseolus vulgaris landraces. The haemagglutination and sugar inhibiting properties differ among the landraces. Sample from Dhanpoo and Darchula show maximum haemagglutinating activity (16HU). The minimum inhibitory concentration of sugar among cultivars was found in the range of 25-50 mM. The haemagglutinating activity of lectins was found to be stable upto temperature of 700C, above which there was no haemagglutinating activity. Similarly the lectin activity was found to be stable at pH range of 7-8, which means haemagglutination activity is negligible in too acidic and basic medium. The crude extract of lectins was subjected for partial purification through ammonium sulphate precipitation and SDS-PAGE analysis. The molecular weight of lectin was found to be 34kd, which is similar to commercially prepared Phytohaemagglutinin used as a standard.
Keywords:-Phytohaemagglutinin, Sugar inhibition, Haemagglutination titer. |
Filename | Singh P Aradhana et.al..pdf |
Filesize | 204 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 04/18/2011 00:00 |
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Last updated on | 05/03/2011 02:57 |
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