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Name | Quantitative Bioanalysis by LC-MS/MS: A Review |
Description | Review article:- Sharma Devanshu, Mittal Rahul, Gupta Annu, Singh Kishan, Nair Anroop* M.M. COLLEGE OF PHARMACY , M.M. UNIVERSITY , AMBALA- 133207, India.
Abstract:- Liquid chromatography-mass spectrometry (LC-MS/MS) is a technique that uses liquid chromatography (or HPLC) with the mass spectrometry. (LC-MS/MS) is commonly used in laboratories for the qualitative and quantitative analysis of drug substances, drug products and biological samples. LC-MS/MS has played a significant role in evaluation and interpretation of bioavailability, bioequivalence and pharmacokinetic data. This article reviews the most recent advances in sample preparation, separation, different types of cartridges used and steps involved in bioanalytical method development and validation of drug molecules. Newly introduced techniques such as ultra performance liquid chromatography (UPLC) with small particles (sub 2 µm) provide better efficiency when compared to other chromatographic techniques. Solid phase extraction (SPE) is the commonly used technique for sample preparation to reduce both time and labor in bioanalysis. Further, this paper also discusses about the matrix effect in LC-MS/MS analysis and how to reduce matrix effect in method development.
Keywords:- LC-MS/MS bioanalysis, sample Preparation, Ultra performance liquid chromatography, matrix effect. |
Filename | Nair Anroop et.al..pdf |
Filesize | 276.51 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/16/2011 00:00 |
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Last updated on | 07/06/2011 05:01 |
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