Property | Value |
Name | HPTLC Method for the Simultaneous Estimation of Atorvastatin, Glimipride and Metformin in Combined Dosage Form |
Description | Original research article:- K.R. Alagawadi, *A.Manikanta Kumar Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, Belgaum, Karnataka, India.
Abstract:- A Simple, sensitive, selective and precise high-performance thin layer chromatographic method for analysis of atorvastatin, glimipride and metformin in pharmaceutical dosage form. The method employed TLC aluminium plates Precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of water: methanol: ammonium sulphate (3.5:3.5:12.6, v/v/v). This system was found to give compact spots for atorvastatin, glimipride and Metformin (Rf value of 0.50 ± 0.01, 0.65 ± 0.01 and 0.33 ± 0.01). Densitometric analysis of atorvastatin, glimipride and metformin were carried out in the absorbance mode at 245nm. The linear regression data for the calibration plots showed good relationship with r2 = 0.999 ± 0.001 from 100-700 ng for atorvastatin, r2 = 0.998± 0.002 from 20-140 ng for glimipride and r2 = 0.996± 0.001 from 100 -1500 ng for Metformin, respectively. The methods were validated for precision, accuracy, ruggedness and recovery. The limits of detection and quantification were 20 and 80 ng per spot for atorvastatin, 5 and 20 ng per spot for glimipride and 50 and 100 ng per spot for metformin, respectively.
Keywords:- High-performance thin layer chromatography (HPTLC), Atorvastatin (ATO), Glimipride (GLI) and Metformin (MRT). |
Filename | Kumar Manikanta A et.al..pdf |
Filesize | 219.03 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/16/2011 00:00 |
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Last updated on | 07/06/2011 06:03 |
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