Property | Value |
Name | Pharmacognostic Standardization of Actaea spicata |
Description | Original research article:- Madaan Reecha*1(M. Pharm.), Bansal Gundeep2 (M. Pharm.), Sharma Anupam3 (Ph.D.) 1.Department of Pharmacy, Faculty of Pharmaceutical Sciences, NIMS University, Shobha Nagar, Jaipur Rajasthan, India. 2.Deparment of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India 3.Pharmacognosy Section, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160 014, India .
Abstract:- Actaea spicata Linn. (Ranunculaceae) has been traditionally used for the treatment of various ailments such as rheumatism, inflammation, nervous disorders, lumbago, scrofula and chorea, but no work has ever been carried out for standardizing this potential plant. The present investigation establishes histological characters, micrometric determinations and physicochemical parameters for A. spicata. Microscopically, root of A. spicata showed the presence of lenticels, two layers of cork cells, 5-6 layers of cortex made up of parenchyma cells containing scattered calcium oxalate crystals, pericycle layer, medullary rays, xylem vessels and phloem parenchyma. Yellowish brown cork, parenchyma cells, scattered rosette shaped calcium oxalate crystals, lignified pitted vessels, pericyclic fibres, and starch grains simple as well as compound (2-7 cells) were observed in powdered microscopy of A. spicata roots. Total ash of A. spicata roots was about 3 and 5 times more than acid insoluble and water soluble ash respectively. Water soluble extractive value of the plant was found to be approximately seven times more than petroleum ether soluble extractive value and slightly higher than alcohol soluble extractive value. Thin layer chromatography of petroleum ether extract showed six spots using hexane : chloroform :: 17 : 3 as mobile phase whereas chloroform extract showed nine spots using toluene : ethyl acetate : glacial acetic acid :: 85 : 15 : 01 as mobile phase on spraying 0.5 % anisaldehyde followed by heating for 2 minutes at 105C. Phytochemically, the plant was found to contain alkaloids, flavonoids, steroids, tannins, proteins and carbohydrates.
Key words:- Actaea spicata, Alkaloids, Ash values, Extractive values, Flavonoids . |
Filename | Madaan Reecha et.al..pdf |
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Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/17/2011 00:00 |
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Last updated on | 07/07/2011 11:28 |
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