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Name | Rapid and Simultaneous Determination of Vincristine and Quercetin in Plasma by Ultra High Performance Liquid Chromatography |
Description | Original research article:- Wong Man-Yi , BSc (Hons); Chiu Gigi N.C. , PhD Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117543.
Abstract:- A reversed phase ultra performance liquid chromatography (UPLC) method was developed and validated to determine plasma levels of vincristine and quercetin simultaneously. Vincristine, quercetin and apigenin (internal standard) were extracted from plasma by liquid-liquid extraction with ethyl acetate. UPLC analysis was carried out at 25 ºC with a Waters AcQuity UPLC BEH C18 2.1 x 50 mm, 1.7 μm column with the gradient elution of water/formic acid (99.9:0.1, v/v) and acetonitrile/formic acid (99.9:0.1, v/v). The flow rate was 0.5 mL/min and the injection volume was 1 μL. Vincristine and quercetin were detected at wavelengths of 297 nm and 376 nm, respectively, with elution time of 1.45 and 1.13 minutes. The calibration curves were linear from 0.156 to 50 μg/mL for vincristine (r2=0.999) and 0.075 to 6.0 μg/mL (r2=1.000) for quercetin. The coefficients of variation (CV) for the intra- and inter-day assays were below 15.5%. The lower limit of quantitation for vincristine and quercetin in plasma were 0.1 μg/mL and 0.02 μg/mL, respectively. Absolute recoveries ranged from 103.2% to 104.8% for vincristine and 96.7% to 120.0% for quercetin. Both drugs were stable for 24 h at 4ºC. This method was successfully applied to determine plasma vincristine, quercetin metabolites and quercetin levels after intravenous administration in mice.
Key words :- Pharmacokinetics, quercetin, UPLC, vincristine. |
Filename | Chiu Gigi N C et.al..pdf |
Filesize | 678.17 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/16/2011 00:00 |
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Last updated on | 07/07/2011 11:59 |
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