Property | Value |
Name | Nitric Oxide Induce IL-10, a CD4+ T Helper Type-2 (Th-2) Cytokines In Human PBMC |
Description | Original research article:- M. Manikannan1, M.Sc., R. Balamurugan1, M.Sc., R. Varatharajan,2 S. Dinesh1, M.Sc., *Elanchezhiyan Manickan1, Ph.D., M.D., 1. Research Scholars Division of Virology and Immunology, Department of Microbiology, Dr. ALM Post Graduate Institute of Basic Medical Science, University of Madras, Taramani, Chennai-600113, India. 1*Professor, Department of Microbiology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai- 600 113, India. 2.Medical officer, Voluntary Health Sciences, Adyar, Chennai- 600 020, India. Abstract:- Background: Nitric oxide and cytokines are produced by the immune cells in response to various stimuli and the initial cytokine milieu at which immune cells interact have a positive impact on the outcome of the immune response generated. Over expression or polarization of a particular cytokine(s) is known for to affect the outcome of the disease. Immunotherapy is a novel approach to combat cytokine mediated diseases and several medicinal plants were shown to possess immune modulatory properties. One of the popular Indian medicinal plants known as Pongamia pinnata (P. pinnata) has been extensively studied for its bioactive properties however, immune modulatory property of the plant extract has not been widely studied. Hence the objective of this study is to study the immune stimulation property of P. pinnata on healthy human PBMCs. Methods: Extracts derived from various solvents were tested for their immune stimulation properties. Briefly, Nitric oxide (NO) production was analyzed upon stimulation of RAW 264.7 cells with plant extract and controls and the supernatants was collected and tested for NO. Similarly culture supernatants obtained at different time points from PBMC stimulated with the extract and were screened for Th-1 and Th-2 cytokines by ELISA. Results: Experiment conducted on RAW 264.7 cells indicated that P. pinnata extract stimulated NO activity. Of the solvents used, NO activity was best seen with aqueous extract. Secondly P. pinnata has induced remarkable production of IL-10. Other cytokines were not stimulated by this extract. Conclusion: Results of our study clearly indicated that P. pinnata extract induced higher levels of Nitric oxide. Maximum NO activity was noticed with aqueous extracts followed by the other extract preparations. It is known that NO can induce Th-2 cytokine and results are in support of this notion.
Key Words:- ELISA, Immune modulation, Immunotherapy, Nitric oxide, PBMC, Pongamia pinnata, Th-1, Th-2 cytokine. |
Filename | Manickan E et.al..pdf |
Filesize | 242.52 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/17/2011 00:00 |
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Last updated on | 07/07/2011 13:20 |
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