Property | Value |
Name | High Performance Thin Layer Chromatographic Analysis of Paracetamol and Etodolac in Spiked Human Plasma |
Description | Research article:- Chaube Poonam H., Gandhi Santosh V. *, Deshpande Padmanabh B., Kulkarni Veena G. Department of Pharmaceutical Analysis, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Pune - 411 001, MH, India.
Abstract:- A new simple, accurate, and precise densitometric method for determination of Paracetamol and Etodolac in spiked human plasma has been developed and validated. A Simple precipitation method was carried out by using methanol and a known amount of supernatant solution was spotted on precoated silica gel 60 F254 plates using a Camag Linomat V autosampler. Detection and quantitation were performed without using an internal standard. The mobile phase selected was Toluene: Ethyl acetate: Methanol (5:4:1, v/v/v) with UV detection at 234 nm. The retention factors for Paracetamol and Etodolac were found to be 0.40 ± 0.05 and 0.64 ± 0.05, respectively. The calibration curve was linear in the concentration range 100 to 600 ng/band for both the drugs in human plasma. The limit of quantitation was found to be 100 ng for both the drugs and no interference was found from endogenous compounds. The % recovery from human plasma using the precipitation method was found to be 93.06 for Paracetamol and 91.97 for Etodolac, respectively. The method provides a direct estimate of the amount of Paracetamol and Etodolac present in human plasma. The method was validated with respect to linearity, accuracy, precision and stability as per the Bioanlytical Method Validation guidelines.
Key Words:- Paracetamol, Etodolac, High Performance Thin Layer chromatography, human plasma. |
Filename | Gandhi Santosh V et.al..pdf |
Filesize | 274.82 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/17/2011 00:00 |
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Last updated on | 07/12/2011 16:47 |
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