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Name | Enhanced Production of Hypericins in Shoot Cultures of Hypericum Perforatum by Interference With Precursor Metabolism |
Description | Research article:- *Dr. Uma Maheswara Rao V, M.Pharm, Ph.D1, Dr. Gaviraj E.N, M.Pharm, Ph.D, . 2 Veeresham C, M.Pharm, Ph.D,PDF,3 1.School of Pharmacy, Nalla Narasimha Reddy Educartion Society’s Group of Institutions, Korremula 'X' Road, Ghatke-sar (Mandal), Ranga Reddy (Dist), A.P, India- 500 088,India. 2.University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India. 3.University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India
Abstract:- The production of hypericins, which are antidepressant, anticancer and anti-HIV constituents of Hypericum perforartum (St. John’s Wort) by in vitro techniques is increasingly considered as an alternate source. Shoot cultures, at different growth phases, were therefore treated with a variety of precursors and organic compounds to stimulate the biosynthesis of hypericins. Since sodium acetate and emodin at 10 mg/l stimulated the accumulation of hypericins at a mid exponential growth phase, dose optimization of these precursors was carried out at the concentrations of 5, 10, 25 and 50 mg/l. A dose-dependent improvement in the yield was seen with sodium acetate. At 50 mg/l, the production of pseudohypericin (236.4 mg/l) increased by 2.4-folds while it was 2.2-folds high for hypericin (17.16 mg/l). However, emodin at 25 mg/l produced the greatest response. The accumulation of pseudohypericin increased by 3.2-fold to 309.7 mg/l and hypericin accumulated up to 25.8 mg/l, which was 2.5-folds more than that of the control. Prominent enhancement in the yields was achieved when sodium acetate and emodin were feeded simultaneously. The accumulation of pseudohypericin and hypericin was elevated, respectively, by 4.5- and 3.7-folds. Among the organic compounds, succinic acid (5 mM) and malic acid (10 mM) stimulated the production of hypericins at a mid exponential growth phase although the effect was moderate. Our study indicated a key role of sodium acetate and emodin in enhancing the accumulation of hypericins in shoot cultures of H.perforatum.
Key words :- Hypericum perforatum, hypericins, organic compounds, precursors, shoot cultures. |
Filename | Rao M Uma et.al..pdf |
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Created On: | 06/17/2011 00:00 |
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