Property | Value |
Name | Optimization of parameters for the production of L-asparaginase by Serratia marcescens |
Description | Original research article:-1D. Praveen Kumar*, 1Thangabalan. B, 1Venkata Ramana. M, 1Vadivel. K, 1Manohar Babu. S , 2 D. Srinivasa Rao 1.SIMS College of Pharmacy, Mangaldas nagar, Guntur-522 001, India. 2.K.C.REDDY Institute of Pharmaceutical Sciences, Jangamguntla Palem, Medikonduru mandal, Guntur 522 348, India.
Abstract:-L-asparaginase is an anti-neoplastic agent used in the lymphoblastic leukemia chemotherapy. In the present study a novel strain, Serratia marcescens- NCIM 2919 was explored for the production of L-asparaginase using citrus limetta pulp. The L-asparaginase enzyme production conditions like incubation period, incubation temperature, particle size, pH, inoculum level and moisture content were optimized. The optimum time, temperature, pH and moisture content for the production of L- asparaginase enzyme were 48hrs, 28oc, 7.5 and 60% respectively. Higher titres L-asparaginases were observed when medium was supplemented with carbon (Sucrose (1.5%) and nitrogen (L-asparagine (0.6%) sources. Under these optimum conditions, maximum production of L-asparaginase was found to be 83.16 U/g.
Key Words:- L-aAsparaginase, Serratia marcescens, citrus limetta pulp, optimization. |
Filename | Kumar Praveen D et.al..pdf |
Filesize | 254.63 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 06/17/2011 00:00 |
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Last updated on | 07/21/2011 16:33 |
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