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Name | A Validated and simplified RP-HPLC of Rizatriptan benzoate from bulk drugs |
Description | Research article:- *Punna Venkateshwarlu1, Srikanth Gajam2 1.Department of Quality Assurance, Nalanda College of Pharmacy, Cherlapally, Nalgonda, A.P, India. 2.Department of Pharmaceutics, Nalanda College of Pharmacy, Cherlapally, Nalgonda, A.P,India.
Abstract-:A simple, specific, accurate, and precise reverse phase liquid chromatographic method(RP-HPLC) was developed and validated for the estimation of Rizatriptan from bulk drugs. A RP : Inertsil ODS 3V (4.6mm x 250mm), 5µm in isocratic mode, with mobile phase containing 8 ml of Phosphate buffer, 58 ml of Acetonitrile and 34 ml of Methanol. The ratio pH was found to be 5.5. The flow rate was 1.0 ml/min and effluents were monitored at 225 nm. The retention time of Rizatriptan was 8.7 min. The linearity of the method was good (r > 0.998), as also were intra-day and inter-day precision (RSD <2%). The method was validated for accuracy, specificity, limit of quantification, limit of detection, robustness and stability. The results showed that proposed method is successfully applied for the quantitative determination of Rizatriptan in bulk drugs.
Key Words:- Reverse phase liquid chromatography, Rizatriptan, HPLC, specificity, validation. |
Filename | Venkateshwarlu Punna et.al..pdf |
Filesize | 267.92 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 07/17/2011 00:00 |
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Last updated on | 08/10/2011 19:47 |
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