Property | Value |
Name | Bioactivity-Directed Separation of an Anxiolytic Fraction from Medicago sativa Linn. |
Description | Original research article:- *Kundan Singh Bora1 (M. Pharm.) and Anupam Sharma2 (Ph.D., M.D.) 1.NIMS University Rajasthan, Shobha Nagar, Jaipur-303 121, India.
2.University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160 014, India.
Abstract:- Medicago sativa Linn. (family: Leguminosae) has a long tradition of use as Ayurvedic and Homoeopathic medicine in variety of central nervous system (CNS) disorders. Traditionally, M. sativa is used to improve the memory, to cure kidney pain, cough, as a rejuvenator, antidiabetic, antioxidant, anti-inflammatory, antimicrobial, and in CNS disorders. Despite a long tradition of use, no systematic phytochemical and pharmacological work has been carried out on this potential plant. The present study has been designed to evaluate the anxiolytic activity of different fractions of bioactive methanol extract of the aerial parts of M. sativa using widely accepted elevated plus-maze model of anxiety in mice. An attempt has been made to isolate the bioactive fraction by resorting to bioactivity directed fractionation and column chromatographic technique. Sub-fraction F4 of bioactive ethyl acetate fraction which was derived from the methanol extract of the plant showed significant anxiolytic activity at a dose of 20 mg/kg, p.o., as compared to the standard drug, diazepam (2 mg/kg p.o.). TLC profile of this sub-fraction comprises of four spots indicating presence of flavonoid components. The study validates the traditional use of M. sativa for the treatment of CNS disorders like anxiety.
Key Words:- Anti-anxiety, Ethyl acetate fraction, Flavonoids, Medicago sativa. |
Filename | Bora S Kundan et.al.-1.pdf |
Filesize | 164.94 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 07/17/2011 00:00 |
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Last updated on | 08/16/2011 14:50 |
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