Property | Value |
Name | LC-MS/MS DETERMINATION OF TRIHEXAPHENYDYL HCl, TRIFLUOPERAZINE HCl AND CHLORPROMAZINE HCl FROM BLOOD PLASMA |
Description | Research article:- * P. Shetti1 , A. Venkatachalam.1 1.Department of Chemistry, Bhavan’s College, Andheri (West), Mumbai – 400058, Maharashtra, India
Abstract:- Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorpromazine HCl is antipsychotic drugs and used for symptomatic treatment of psychotic ailment. We present a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method developed and validated for the quantification of Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorpromazine HCl in Human Plasma. Ornidazole was used as the internal standard. The simple and cost effective Liquid-Liquid Extraction method is applied for the extraction of all three drug and Internal standard from Plasma. A rapid isocratic separation is achieved by a short HYPERSIL, Hypurity C18 (50x4.6)mm 5µ (Thermo, USA) column using mobile phase of Acetonitrile and Ammonium acetate buffer (70:30 v/v, (%) ) at flow rate of 0.4 ml/min. The run time is 2 minutes suggests high throughput of the proposed method. . The compound was ionized in the positive electro spray ionization (ESI) mode of the mass spectrometer. Detection of mass was performed in multiple reactions monitoring (MRM) mode; Trihexyphenidyl m/z 302.2-►98.1, Trifluoperazine m/z 408.1-►113.1, Chlorpromazine m/z 319.1-►86.1 and Ornidazole 220.1-►128.1. The response for all the three drugs found to be linear over the range of 0.1-100 ng/ml. The lower limit of quantification of the LC-MS/MS method was 0.1ng/ml. The intra-and inter-day precisions were below 10.0% and the accuracy was between + 5.0% and - 5.0%. This validated LC-MS/MS is rapid, sensitive, specific and cost-effective method for determining Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorporomazine HCl in human plasma samples.
Key words :- Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorpromazine HCl, LC-MS/MS study, Bioequivalence, Development and validation. |
Filename | Shetti P et.al..pdf |
Filesize | 364.71 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 08/16/2011 00:00 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 7667 Hits |
Last updated on | 09/04/2011 19:01 |
Homepage | |
CRC Checksum | |
MD5 Checksum |