Property | Value |
Name | Optimization of L-asparaginase Production By Streptomyces griseoluteus WS3/1 Using Experimental Methods |
Description | Research article:- P.V.Kamala Kumari*, G.Girija Sankar, T.Prabhakar Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
Abstract:- Aim: Optimization of different process parameters for L-asparaginase production by Streptomyces griseoluteus WS3/1 and its validation using response surface methodology was carried out during the present study. Methods and results: Four medium factors were screened by conventional methods and subsequent optimization process using Central composite design (CCD) was performed to find out the optimium values of the selected parameters. Dextrose, L-asparagine, pH and inoculum level were found to be the best medium factors for the optimization of L-asparaginase production and central composite design experiments indicated the optimal values of dextrose, L-asparagine, pH, inoculum level were 0.45 % w/v, 1.162 % w/v, 7.4 and 10.36 % v/v respectively. Conclusions: The combination of conventional method with central composite design (CCD) was effective and reliable in selecting the statistically significant factors and determining the optimal levels of those factors in fermentation medium. This work demonstrates the usefulness of CCD for determining the optimum conditions for maximizing L-asparaginase production. Significance and impact of the study: The combined optimization method described here is the effective for screening medium factors as well as determining their optimum levels for the production of L-asparaginase by Streptomyces griseoluteus WS3/1.
Key words :-S.griseoluteus WS3/1, L-asparaginase, CCD. |
Filename | Kamala Kumari P .V et. al..pdf |
Filesize | 296.5 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 09/17/2011 00:00 |
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Maintained by | Editor |
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Last updated on | 10/10/2011 02:34 |
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