Property | Value |
Name | High performance liquid chromatographic method for evaluation of two antibiotic residues in liver and muscle of broilers in Ibadan city, Southern Nigeria. |
Description | Research article:- 1 Adewuyi, G. O.,* 2 Olatoye, O. I., 1Abafe, A.O., 1 Otokpa, M. O. and 1 Nkukut, N. K. 1.Department of Chemistry, University of Ibadan, Nigeria. 2.Department of Veterinary Medicine, University of Ibadan, Nigeria.
Abstract:- The extensive use of some antibiotics for broilers in Nigeria has been a major practice in recent times. The purpose is to enhance the health and productivity of flocks. The use of antimicrobials is strictly restricted by the Food and Drug Administration and USDA to warrant their safety and efficacy. These drugs are retained as residues in tissues and livers of ready for table poultry meats, long after their application. Some residues have been implicated as potential carcinogenes in human. In our study, a method for determination of residual chloramphenicol and oxytetracycline in livers and tissues of broilers using High Performance Liquid Chromatographic Technique has been established and validated. Chloramphenicol and tetracycline were extracted from livers and tissues by standard procedures and clean-up by liquid-liquid extraction after which extracts were analysed by HPLC. From the result, the recovery values for samples analysed ranged between 76.4 to 85% for all samples analysed. Chloramphenicol residue level in breast tissue and liver ranged from 89.33±3.00 to 223.05±2.76 μg/Kg and 118.05±2.05 to 415.00±7.64 μg/Kg. Oxytetracycline residue level in breast tissue and liver ranged from 670.00±3.00 to 1816 ± 49.33 and 1551.16±10.6 to 2697.53±1.89 μg/Kg. The coefficients of variation obtained were less than 10% which is an indication of high precision. The limit of detection for oxytetracycline and chloramphenicol was 13.0 and 7.0 μg/Kg while the limit of quantification was 42.0 and 24.0 μg/Kg respectively. The residual concentrations of the antibiotics were higher than the maximum residue limits established for them by the European Union (EU) which is 100μg/Kg for oxytetracycline and zero for chloramphenicol.
Keywords:- Antibiotics, Carcinogenic, Euthanise, Recovery, Extraction. |
Filename | Adewuyi G O et. al..pdf |
Filesize | 147.33 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 10/16/2011 00:00 |
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Maintained by | Editor |
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Last updated on | 11/13/2011 18:58 |
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