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Name | Phenotypic Characterization of Candida species and their antifungal susceptibility from a tertiary care centre |
Description | Research article:- * Dr.Binesh Lal.Y1, Dr.Kalyani.M2. 1.Assistant Professor, Department Of Microbiology, Saveetha Medical College,Thandalam, Kancheepuram District Tamilnadu, India. 2.Professor, Department Of Microbiology, Saveetha Medical College,Thandalam, Kancheepuram District, Tamilnadu, India.
Abstract: -Back ground and objective: With the increase in the number of immunocompromised individuals, there has been a consequent rise in the number of opportunistic infections, especially those due to Candida species. A rise in the incidence of antifungal resistance has also been reported. Aim: To isolate and identify the Candida species causing infections in patients from a tertiary care centre and also to study their antifungal susceptibility profile. Materials and methods: One hundred and twenty seven candidial isolates from urine (73), vaginal swabs (24), exudates (21) and blood (09) were taken for the study. The four species of Candida isolated in this study were Candida tropicalis 54.3%, C.albicans (37.8%) C. paropsilosis (5.5%) and C.glabrata (2.4%). Antifungal susceptibility tests against Amphotericin B, Ketaconazole, Itraconazole, and Fluconazole were done recommended by the Clinical and laboratory standards (CLSI). The zone of inhibition of fungal growth was measured and compared with standard strains as per manufacturer’s instructions. Results: Out of 127 isolates, the commonest source of isolation of candida species in the decreasing order of frequency, namely urine 57.5%, vaginal swab (18.9%), exudates 16.5% and blood 7.1%. Candida tropicalis 54.3% was the predominant isolate followed by C.albicans 37.8%. C.parapsilosis 5.5% and the least common isolate C.glabrata 2.7%. Maximum numbers of the candidial isolates 57.5% were from urine samples and urinary catheterization was noted as risk factor among urinary samples. CHROMagar candida medium is found to be useful in identification of Candida species. All the candidial isolates were susceptible to Amphotericin-B. C.albicans 6.3% and C.tropicalis 37.7% showed higher degree of resistance to Fluconazole. C.parapsilosis 57.1% to Ketaconazole and C.glabrata to Ketaconazole 66.6% and Fluconazole 66.6%.
Keywords:- Candida species, 10% Potssium hydroxide (KOH), Sabouraud’s Dextrose Agar, CHROMAgar. |
Filename | Binesh Lal Y et.al..pdf |
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Creator | admin |
Created On: | 10/17/2011 00:00 |
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Last updated on | 11/14/2011 09:03 |
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