Property | Value |
Name | Isolation and optimization of metabolite production from mutant strain of Bacillus sp. with antibiotic activity against plant pathogenic agents |
Description | Research article:- *Kunal Madhav1, Satish Verma2, Ritu Tanta3 .
1Lecturer, Department of Microbiology, Sai Institute of Paramedical And Allied Sciences, Dehradun, Uttarakhand, India. 2Lecturer, Department of Biotechnology, Sai Institute of Paramedical And Allied Sciences, Uttarakhand ,India. 3Department of Microbiology , Sai Institute of Paramedical And Allied Sciences, Dehradun, Uttarakhand, India.
Abstract:- The present work is focused on the isolation of Bacillus spp. from the soil sample of industrial, city and agricultural land in Dehradun (Uttarakhand). The collected samples were labeled as Agricultural soil sample (ASB), Industrial soil sample (ISB) and city soil sample (CSB). Soil samples were then serially diluted in normal saline and plated on sterile nutrient agar plates. The colonies obtained from higher dilutions were subjected to Gram staining, biochemical reactions and starch hydrolysis test. Bacterial colony yielding positive starch hydrolysis test were subjected to Amylase activity test. The amylase activity was also carried on the isolated bacteria with respect to time, temperature and pH of the media in which it was inoculated. The result showed that the maximum amylase activity in case of Agricultural soil (34.2%) followed by city sample (26.07%) and industrial sample (21.79%). Amylase activity was also checked with respect to temperature, time and pH. The activity of amylase was seen to increase with respect to time, pH and temperature which was found to be 18.33 IU, 6.89 IU and 6.85 IU in case of Agricultural soil bacilli respectively; whereas both city and industrial soil sample yielded comparatively low results.
Keywords:- Amylase activity, Bacilli, Soil, pH effect. |
Filename | Madhav Kunal et al..pdf |
Filesize | 462.4 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 11/16/2011 00:00 |
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Maintained by | Editor |
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Last updated on | 12/17/2011 08:08 |
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