Property | Value |
Name | Acute and Repeated Dose 90-Day Oral Toxicity Studies of Clausena excavata Extract in Rats |
Description | Research article:- * Kunanusorn Puongtip1, Panthong Ampai2, Nuntasaen Narong3, Pittayanurak Prapadsorn4, Reutrakul Vichai5 .
1 Ph.D., Department of Pharmacology and Center for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 2 Dr. rer. nat., Department of Pharmacology and Center for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 3 M.Sc., The Forest Herbarium National Park, Wildlife and Plant Conservation Department, Ministry of Natural Resources and Environment, Bangkok, Thailand. 4 B.Sc., 5 Ph.D., Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Abstract:-Clausena excavata Burm. f. has been used in Thailand as a folk medicine for the treatment of several disorders. To evaluate its safety, toxicity studies of the methanol extract from the stems of C. excavata were performed in rats. The extract was administered orally at a dose of 5,000 mg/kg in acute toxicity study, and at doses of 50, 100, and 200 mg/kg/day for 90 days in repeated dose 90-day toxicity study. Toxicological parameters were examined at the end of each study. Student's paired t-test was used to analyze data obtained from acute toxicity study. One-way analysis of variance and post hoc least-significant difference test were used to determine statistical difference between groups in the repeated dose 90-day toxicity study. In acute toxicity study, the extract caused no signs of toxicity, death or any pathological abnormalities at necropsy on day 14. Thus its oral LD50 is suggested to be greater than 5 g/kg. In the repeated dose 90-day toxicity study, no signs of toxicity and death were observed. Some hematological and biochemical parameters as well as some internal organ weights showed statistically significant changes when compared with those of the control group. All parameters of the treated rats were within the normal laboratory ranges for the Sprague-Dawley rats and were considered as biological variations. Moreover, no abnormality of all organs examined was observed in both gross and histopathological examinations. These results suggest that the no-observed-adverse-effect level of the extract is 200 mg/kg/day following daily oral administrations for 90 days.
Keywords:- Acute oral toxicity, Clausena excavata, 90-day oral toxicity, Rats, Stem extract. |
Filename | Puongtip Kunanusorn et. al..pdf |
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Created On: | 11/16/2011 00:00 |
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Last updated on | 12/16/2011 18:14 |
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