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Name | The Prevalence of Cryptosporidiosis in HIV Seropositive individuals and detection of Cryptosporidium parvum Oocysts by Modified Ziehl-Neelsen staining, Giemsa staining and Sheather sugar flotation technique in a Tertiary Care Hospital |
Description | Research article:- *Dr. Narayan Shrihari MD (Microbiology)1, Dr. KumudiniT.S D.Bact (Microbiology) 2, Dr. Mariraj. J MD (Microbiology) 3, Dr. Krishna.S MD (Microbiology) 4 1Asst. Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 2 Tutor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 3 Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 4 Professor and Head, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
Abstract:- Background: Diarrhoea is a common complication of infection with HIV leading to weight loss and cachexia. It occurs in almost 90% of the HIV patients. Cryptosporidium parvum is a major cause of diarrhoea in developing countries, mainly affecting children and HIV infected individuals with low CD4 counts. The infection is self limiting in immunocompetent hosts but can be severe and persist in the immunocompromised and malnourished individuals. Aims: To know the prevalence of Cryptosporidiosis in HIV patients attending our Hospital and detection of Cryptosporidium parvum oocysts by modified Ziehl-Neelsen staining, Giemsa staining and Sheather sugar flotation technique. Settings and Design: A retrospective study for a period of one year Material and Methods: Stool samples from 51HIV patients with diarrhoea and one HIV patient without diarrhoea were examined for oocysts by modified Ziehl-Neelsen(ZN) staining, Giemsa(GS) staining and Sheather sugar flotation (SSF) technique. Results: Of the 52 stool samples, modified ZN staining detected 14 (26.92%), GS staining12 (23.07%) and SSF technique 17 (32.69%) stool samples positive for oocysts of Cryptosporidium parvum. Conclusions: There is a high prevalence of cryptosporidiosis in HIV infected patients and simple method modified ZN staining can detect oocyst in stool sample.
Keywords:- Oocysts, Giemsa staining, Sheather sugar flotation, Cryptosporidium parvum. |
Filename | Shrihari Narayan et. al.(1).pdf |
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Creator | admin |
Created On: | 12/16/2011 00:00 |
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Last updated on | 01/10/2012 08:14 |
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