Property | Value |
Name | Copper Induced Antioxidative Enzyme Indices in Leaves of Brassica juncea Seedlings. |
Description | Research article:- *R.C.Pantola1, G.S.Shekhawat2 1Research scholar, Department of Biosciences and Biotechnology, Banasthali University, Banasthali 304022, Rajasthan, India. 2Associate Professor, Department of Biosciences and Biotechnology, Banasthali University, Banasthali 304022, Rajasthan, India.
Abstract:-In vitro grown seedlings of Brassica juncea were treated with 0.5, 1.0, 5.0, 10.0, 20.0, 40.0, 50.0, 75.0, and 100.0 µ mol concentration of copper and compared with non- treated control. Antioxidative enzymes (superoxide dusmutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)) indices of leaves were studied by analyzing these antioxidative enzymes in day 5 and 10. The seedlings were grown on Hoagland’s nutrient solution. Our results indicate an enhancement in the activity of APX in response to copper stress at 10 µ mol on day 5 and 1, 5,10,40,50 and 75 µ mol copper treatment on day 10. While the APX activity decreased at 1,5,20,40,50,75 and 100 µ mol on day 5 and 0.5,20,100 µ mol on day 10. CAT activity increased at 5,10,40,50 and 75 µ mol copper concentration on day 10 , at 10 and 20 µ mol on day 5,while it was moderate at 0.5,40,50,75, and 100 µ mol on day 5 and 0.5 and 1 µ mol on day 10. CAT activity was decreased on day 5 at 0.5 and 20 µ mol and 100 µ mol on day 10, with respect to untreated control. SOD activity gradually decreased up to 5 µ mol and increased at 10 µ mol but at 20, 40, 50 and 75 µ mol it was observed slightly, gradual increment, at 100 µ mol it was drastically high that is more than double of control. On day 10, SOD activity was found less than control up to 10 µ mol, at 20 µ mol it was minimal and again at 40, 50, 75 and 100 µ mol it remains somehow equal but less than control.
Key Words:- Antioxidant enzymes. Brassica juncea, Copper, Oxidative stress. |
Filename | Pantola R C and Shekhawat G.S..pdf |
Filesize | 222.5 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 02/17/2012 00:00 |
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Last updated on | 03/20/2012 12:01 |
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