Property | Value |
Name | Extraction and detection of the quarternary alkaloids of the stem bark of Alstonia boonei plant (family: apocynaceae), by the sulphonephthalein dye-stuff ion-pair complexation at pH 5.58. |
Description | Original research article:- *Oguegbulu, N. Edwin1, Uche, I.Fidelia2 1Department of Pharmaceutical and Medicinal Chemistry,Faculty of Pharmaceutical Sciences, University of Port Harcourt, Rivers, State Nigeria. 2Department of Pharmacognosy and Phytomedicine, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Rivers, State Nigeria.
Abstract:- Background and aim: Alkaloids as vegetable alkalis found often in higher plants, are however occasionally present also in lower plants and in animals. They are indeed very medicinally useful secondary metabolites and can occur as primary, secondary, tertiary or quaternary compounds. The extractability of quaternary alkaloids as in N-oxides tends to pose a serious challenge to natural products Chemists, especially in the resource - limited countries. This is because of their high level of polarity and hence a significant affinity to the aqueous-acid phase usually employed in the common extraction process. Alstonia boonei, a proven versatile medicinal plant used in African traditional medicine has been confirmed to contain a heavy load of alkaloids characteristics of which are consistent with the already established quaternary alkaloids of the other members of the Alstonia species. The objective of this research is to address these highlighted challenges posed by the extractability complexity of quaternary alkaloid and to aid in the quick identification of the same in the far away wood. Methodology: Complexation of these quaternary alkaloids with bromocresol purple – a sulphonephthalein dye, at a specific pH of 5.58 was achieved and subjected to a comparison with Zn-reduction extracts of same using tlc techniques. Results: The observations have led to a prospect for enhanced and quick extractability of quaternary alkaloids of this plant using simple facilities of; TLC, UV – lamp and chromatographic colour reactions for the detection. Conclusion: The dye stuff-complexation extracts have compared favourably with the extraction product of Zn-reduction technique already established for the quaternary alkaloidal extraction. Development of this method can then be a fast, simple and reliable innovation in the extraction of quarternary alkaloids of Alstonia boonei.
Key Words:- Alstonia boonei plant stem bark, quaternary alkaloids, Zn reduction complexes,bromocresol ion-pair complexation extracts at pH 5.58, tlc detection. |
Filename | Oguegbulu N Edwin and Uche I Fidelia..pdf |
Filesize | 55.4 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 02/18/2012 00:00 |
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Maintained by | Editor |
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Last updated on | 04/04/2012 19:23 |
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