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Name | Validated HPLC Method for Simultaneous Quantitation of Amlodipine Besylate, Atenolol and Aspirin in Bulk Drug and Formulation |
Description | Original article:- Bhusari Vidhya K. 1, Dhaneshwar Sunil R. 2* 1Ph.D. Scholar, Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India 411038. 2Professor & Chairperson, Department of Pharm.Chemistry,RAK Medical & Health Sciences University, College of Pharmaceutical Sciences, Ras Al Khaimah Post Box No 11172,U.A.E.
Abstract:- HPLC method has been described for simultaneous determination of Amlodipine besylate, Atenolol and Aspirin in formulation. This method is based on HPLC separation of the three drugs on the Thermo Hypersil BDS–C18 (250 mm × 4.6 mm, 5.0 µ) from Germany with isocratic conditions and simple mobile phase containing methanol: 10 mM phosphate buffer with pH 7.0 adjusted with ortho phosphoric acid (70: 30) at flow rate of 1 mL/min using UV detection at 235 nm with Rt of 2.58 min for Amlodipine besylate, Rt of 3.40 min for Atenolol and 4.23 min for Aspirin. This method has been applied to formulation without interference of excipients of formulation. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 2-12 µg/mL for Amlodipine besylate, Atenolol and 4-24 µg/mL for Aspirin, respectively. The mean values of the correlation coefficient, slope and intercept were 0.9993 ± 0.63, 2134.1 ± 0.54 and 1676 ± 0.89 for Amlodipine besylate, 0.9994 ± 0.91, 21326 ± 1.02 and 42960 ± 0.74 for Atenolol and 0.9993 ± 1.02, 15182 ± 0.48 and 64910 ± 0.64 for Aspirin, respectively. The method was validated for precision, robustness and recovery. The limit of detection (LOD) and limit of quantitation (LOQ) was 0.5 μg/mL and 1 μg/mL for Amlodipine besylate and Atenolol and 1 μg/mL and 2 μg/mL for Aspirin, respectively. Statistical analysis showed that the method is repeatable and selective for the estimation of Amlodipine besylate, Atenolol and Aspirin.
Key Words:-Amlodipine besylate, Aspirin, Atenolol, HPLC, Method development, Validation. |
Filename | Dhaneshwar Sunil R and Bhusari Vidhya K.pdf |
Filesize | 149.42 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 04/17/2012 00:00 |
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Maintained by | Editor |
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Last updated on | 05/20/2012 09:25 |
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