Property | Value |
Name | CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY PATTERN OF NONFERMENTING GRAM NEGATIVE BACILLI FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL, BELGAUM |
Description | Original research article:- *Shilpa.K.Gokale1, S. C. Metgud2. 1Asst. Prof, Dept of Microbiology, SN Medical College, Bagalkot.587102, India. 2Professor, Dept of Microbiology, JNMC, Belgaum, India.
Abstract:- Introduction: -Nonfermenters are being isolated from various clinical specimens. Although frequently considered as contaminants, the pathogenic potential has been proved beyond doubt by their frequent isolation from clinical material and their association with disease. They exhibit resistance not only to the beta lactam and other group of antibiotics but also to carbapenems. Objectives: To characterize the nonfermenters isolated from various clinical samples. To assess their clinical significance. To know their anti microbial sensitivity pattern. Materials and methods: The present study was conducted for a period of one year from Jan 2007 – Dec 2007 at J.N Medical College, Belgaum. The nonfermenters were identified using a standard protocol, which included tests for motility, oxidase production, oxidation-fermentation (OF) test for various sugars, DNAse test and ONPG test. The clinical significance was assessed using various criteria and the susceptibility testing was done by Kirby bauer disc diffusion method. Results: From a total of 130 NFGNB, Pseudomonas aeruginosa was the commonest nonfermenter accounting for 82.3% followed by Acinetobacter baumanii (15.4%). Other significant NFGNB isolated were: Acinetobacter lwofii (0.76%), Burkholderia pseudomallei (0.76%) and Moraxella species (0.76%). Pseudomonas aeruginosa showed good sensitivity to meropenem (96.2%), ciprofloxacin (50%) and amikacin (49.5%). Acinetobacter baumanii showed 96.2% sensitivity to meropenem and 45% sensitivity to ciprofloxacin. Conclusion: P aeruginosa and A baumanii were the common NFGNB isolated in our study. They were associated with various health care related infections like urinary tract infection, blood stream infections, surgical site infections and ventilator associated pneumonia. P aeruginosa showed good sensitivity to meropenem, ciprofloxacin and amikacin. A.baumanii showed good sensitivity to meropenem and ciprofloxacin. The susceptibility pattern of NFGNB varied depending upon the species. Therefore it is essential to identify the nonfermenters to know their trends in sensitivity.
Keywords:-Nonfermenters, P. aeruginosa, A. baumanii, Meropenem, Ciprofloxacin. |
Filename | Gokale K. Shilpa and metgud C.S.pdf |
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Created On: | 04/17/2012 00:00 |
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Last updated on | 05/24/2012 09:39 |
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