Property | Value |
Name | Stability-Indicating Spectrophotometric Methods for The Determination of Ofloxacin and Ceftriaxone and Their Degradation Products |
Description | Original research article:- *Ekram M. Hassan1, M.S. Mahrous2, Ruba N. Shdeed2 1 Department of Pharmaceutical Analytical Chemistry and Quality Control. 2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy Beirut Arab University, Beirut,Lebanon.
Abstract:- Stability indicating, rapid and reliable derivative, absorption difference and derivative difference spectrophotometric methods were developed for the determination of ofloxacin and ceftriaxone in presence of their degradation products. The linear calibration graphs for ofloxacin were obtained from D1 at 281 nm for the acid solutions, D2 at 288.5 nm for the alkaline solutions, ΔA at 298.6 nm, ΔD1 at 292-304.2 nm, and ΔD2 at 298 nm, while those for its corresponding degradation product were obtained from D2 at 281.5 nm of the acid solutions, and D1 and D2 at 288.5 nm and 297 nm, respectively, for the alkaline solutions. On the other side, ceftriaxone was quantified using D2 amplitudes of the solutions in water at 241.3 nm, D1 at 268.3 nm and 238.5 nm, D2 at 257 nm and 283.4 nm, and D3 at 269.7 nm and 298.3 nm, for the acid solutions, while its corresponding degradation product was determined by measuring the D1 and D2 of the solutions in water at 241 nm and 249.8 nm, respectively, D1 and D3 of the acid solutions at 263 nm for both, D1 at 242.2 nm and 270.3 nm, and D2 at 248.7 nm of the alkaline solutions. The proposed methods were successfully applied to the determination of ofloxacin and ceftriaxone in synthetic mixtures with the corresponding degradation products. The results obtained indicated the high accuracy, calculated as mean percentage recovery, RSD% and Er% and precision; evaluated in terms of inter-day and intra-day precision. In addition, ofloxacin capsules and ceftriaxone ampoules were assayed using the proposed methods and the results obtained were compared with reference spectrophotometric methods.
Keywords: Ofloxacin, ceftriaxone, derivative, absorption difference, derivative. |
Filename | Ekram M Haasan et al.pdf |
Filesize | 383.66 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 05/17/2012 00:00 |
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Maintained by | Editor |
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Last updated on | 06/08/2012 11:16 |
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