Property | Value |
Name | Detection of ESBL and Cephalosporinase in Urinary Tract Isolates |
Description | Original research article:-Microbiology and Biochemistry K.Nalini 1*, P.Sumathi 2 1Department of Microbiology , Ayya Nadar Janaki Ammal College, Sivakasi ,Tamil Nadu,India. *Part time Research Scholar, Bharathiyar University, Coimbatore. 2Department of Biochemistry, Government College for Women, Krishnagiri, Tamil Nadu, India.
Abstract:- Background: Antimicrobial resistance is one of the major problem in medicine field faced by humans. Among different mechanisms of resistance beta-lactamase induced resistance plays a prominent role and threatens the therapeutic efficacy of antibiotics. The aim of this study is to detect the presence of ESBL and AmpCase in urinary isolates and also to focus the importance of antibiotic surveillance programs. Methods: During the period of January 2010 to December 2011 Six hundred and fifty six midstream urine samples were collected from different hospitals and clinical lab surrounding Srivilliputtur, Rajapalayam, Madurai and Sivakasi in TamilNadu, India. From this 606 isolates were selected including E.coli (356), Pseudomonas spp (123), Klebsiella spp (73) and Proteus spp (54) species for further analysis .Antibiotic sensitivity pattern were analysed with selected beta-lactam antibiotics. Phenotypic and Biochemical characterisation of selected isolates were performed followed by genotypic detection of blaTEM and blaSHV genes in E.coli. Result: All the isolates were found to be resistant to penicillin and 90% of isolates showed resistance to Amoxicillin followed by variable rate of resistance to second, third and fourth generation Cephalosporin antibiotics and less than one percent of resistance was observed to imipenam. E.coli and Pseudomonas spp showed more potent activity of beta-lactamase. Beta lactamase genes blaTEM and blaSHV genes were analysed in one E.coli isolate. Conclusion: Antibiotic surveillance programs can have a great impact to tackle the problem of antimicrobial resistance which is extending in diverse forms with local, regional and national level variations.
Keywords:-Antimicrobial resistance, beta-lactam antibiotics, ESBL, Cephalosporinase. |
Filename | K Nalini and P Sumathi.pdf |
Filesize | 181.03 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 08/05/2012 15:46 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 4001 Hits |
Last updated on | 08/05/2012 15:51 |
Homepage | |
CRC Checksum | |
MD5 Checksum |