Property | Value |
Name | DNA shielding property of tea (Camellia sinensis) leaf extracts against reactive oxygen species |
Description | Research article:-Biotechnology *1Singh Mukesh (Ph.D), 2Majumdar Anurima (Ph.D), 3Momi Navneet (M.S, Ph.D pursuing) 4Bhattacharjee Aishwarya (M.Tech), 4Majumdar Kaustav (Ph.D pursuing), 4Kundu Soumyabrata (M.Tech), 4 Paul Rituparna (B.Tech), 4 Maity Dipanjana (B.Tech), 1Sasmal Kankaayan (B.Tech pursuing) and 1Sarkar Ankita (B.Tech pursuing).
*1Department of Biotechnology, Haldia Institute of Technology, Purba Medinipur, Haldia, India. 2University of Florida, Gainesville, FL. 3University of Nebraska, Omaha, Nebraska. 4Department of Biotechnology, Bengal College of Engg. & Technology, Durgapur, India.
Abstract:- Free radicals can cause damage to biomolecules and thus alter biological metabolism leading to dreadful diseases like cancer, diabetics, and age related disorder. From ancient times, plants have been known to possess medicinal properties and hence they have immense therapeutic potential. Tea (Camellia sinensis L.) is one of the most common beverages and people all over the world consume it on daily basis. Tea has many bioactive components like polyphenols, catechins, flavonoids etc. that have the intrinsic power to scavenge reactive oxygen species. The present research was undertaken to measure the effects of tea leaves’ extracts in reducing the oxidative stress induced by Mohr’s salt (Fe 2+) ions and H2O2 (Fenton system) in Calf thymus (CT) DNA. In addition, superoxide scavenging activity of the same extracts was also measured by auto-oxidation of hematoxilin. Boiled aqueous extracts of fresh green leaves and semi-fermented leaves were used for the above said study. This study showed that there was a dose-dependent protection of Fe 2+ ions and H2O2 stressed CT DNA and inhibition of superoxide radicals. Both gel electrophoresis and superoxide assay results correlated each other. These results suggested that tea leaves have the potential to serve as a beverage as well as a medicine against free-radical-associated oxidative damage and related degenerative diseases involving metabolic stress, genotoxicity and cytotoxicity.
Key words:- Antioxidant activity, Camellia sinensis, DNA strand, reactive oxygen species (ROS). |
Filename | Singh M et al..pdf |
Filesize | 241.84 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 09/04/2012 00:00 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 2435 Hits |
Last updated on | 09/07/2012 12:56 |
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