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Name | Catechins and Caffeine Contents of Green Tea Commercialized in Thailand |
Description | Research article:- *,1Dr. Jankana Burana-osot and 2Dr. Wandee Yanpaisan 1Associate Professor, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, NakhonPathom 73000, Thailand. 2Assistant Professor, Department of Pharmacognosy, Faculty of Pharmacy, Silpakorn University, NakhonPathom 73000, Thailand.
Abstract:- A validated reversed-phase HPLC methodhas been employed for the determination of five individual catechins; (-) epigallocatechin gallate (EGCG), (-) epigallocatechin (EGC), (-) epicatechin gallate (ECG), (-) epicatechin (EC), and (+) catechin (C), and caffeine in green tea simultaneously.The separation was performed on a C18-bonded silica column (4.6 x 250 mm, 5μm) using an isocratic mobile phase comprising of 0.1% acetic acid and methanol (70:30, v/v) with a UV detection at 230 nm. The method validation showed good results for specificity, linearity, precision, accuracy, limit of detection and limit of quantitation. The content of 5 catechins and caffeine in commonly consume green tea brands in Thailand were determined and compared. The resulting data revealed extensive variability in catechins and caffeine amounts. The quantities of EGCG ranged from 5.19 to 58.21, EGC from 2.80 to 52.48, EC from 0.74 to 11.58, ECG from 1.01 to 16.45 and C from 0.09 to 6.10 mg/g of dry tea. Caffeine contents were between 5.81-27.62mg/g of dry tea. EGCG was in the highest concentration in almost tea infusions excepting Japanese green tea, while C was the lowest, excepting Chinese green tea.
Key words:- Green tea, Catechins, Caffeine, HPLC. |
Filename | Jankana and Wandee.pdf |
Filesize | 457.07 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 09/04/2012 00:00 |
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Maintained by | Editor |
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Last updated on | 09/18/2012 03:22 |
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