Property | Value |
Name | FTIR and UV-Visible spectrophotometeric analyses of artemisinin and its derivatives |
Description | Research article:-Biochemistry Lawal, A*1., Umar, R. A1.,Abubakar, M. G1., Faruk, U. Z2. and Wali, U3 1Department of Biochemistry, 2Department of Pure and Applied Chemistry, 3Chemical Pathology Department, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto-Nigeria.
Abstract:- FTIR is a simple, reproducible technique and most widely used methods for structural elucidation of many chemical compounds, and has been used as a requisite method to identify medicines in different pharmacopoeias. Pellets were prepared by taken 0.150 g of Potassium Bromide (KBr) and mixed with 0.008 g of the reference standards of artemisinin and its derivatives artesunate each and the spectra were recorded between 4000 cm-1 and 500 cm-1, by averaging 10 scans for each spectrum with a Shimadzu FT-IR Spectrophotometer. The fingerprints obtained exhibit the presence of C – H bend, C = O stretch, aromatic C – H in plane; cyclic ethers, large rings, C – O stretch and peroxides, C – O – O – stretch respectively. Additional IR-bands were observed of CH2 – CH2, cyclohexane ring vibration, and ester in artesunate. Also, simple and sensitive Spectrophotometric methods of these antimalarial were developed using methylene blue and soluble starch as chromogenic agents in pure forms of artemisinin (ATM) and artesunate (ATS) and in formulations. The results obtained for calibration curve show that r2 were 0.9602 and 0.9996 for ATM and using methylene blue while 0.9537 and 0.9648 for ATM and ATS using soluble starch as chromogenic agent respectively. The intra- and inter-day precisions were within 48.7 to 96.6% relative standard deviations. Limits of detection and quantification were found to be within accepted range. In post-developmental and validation phases, drugs were sample and tested and the results reveal percent content of API of the drugs between 78 -105% for spectrophotometric methods. Conclusively, the results showed that the proposed methods could be used in determine these antimalarial in formulations.
Key words:- IR fingerprints, methylene blue, soluble starch, artemisinin and artesunate. |
Filename | Lawal et al.(6-14).pdf |
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Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 11/04/2012 00:00 |
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Last updated on | 11/20/2012 14:27 |
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