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Name | RP-HPLC Method Development and Validation for Quantitative Estimation of Metoprolol Succinate and Telmisartan in Bulk Drug and Their Dosage Forms |
Description | Original article:-Pharmaceutical sciences Jain Nilesh *, Sharma Bhupendra Kumar, Jain Ruchi, Jain Deepak Kumar & Jain Surendra. *Sagar Institute of Research & Technology-Pharmacy,Ayodhya Bypass Road, Bhopal, M.P-462041, India.
Abstract:- A simple, precise, rapid and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) method is developed for the simultaneous estimation of Metoprolol Succinate (METO) and Telmisartan (TELM) present in multicomponent dosage forms. Chromatography is carried out isocratically at 25°C ± 0.5°C on an Prontosil C18 Column (5 µm,250mm x 4.60mm) with a mobile phase composed of acetonitrile: methanol: phosphate buffer pH-5 (35:35:30 % v/v/v) at a flow rate of 1.0 mL/min. Detection is carried out using a UV-PDA detector at 225 nm. Parameters such as linearity, precision, accuracy, recovery, specificity and ruggedness are studied as reported in the International Conference on Harmonization guidelines. The retention times for METO and TELM are 2.57 ± 0.5 min.and 4.68 ± 0.5 min. respectively. The linearity range for METO and TELM are 5-25g/ml & 8-40µg/ml and the recovery of added standards (80%, 100% and 120%) was in ranging from 98.24 to 99.35% for METO and 98.21 to 99.74% for TELM. The correlation coefficients for all components are close to 1. The relative standard deviations for three replicate measurements in three concentrations of samples in tablets are always less than 2%. The result obtained shows the developed method to be new (no method available for combination these drugs), rapid (Short retention time), simple, accurate (the value of SD and % RSD less then 2), precise and can be successfully employed in the routine analysis of these drugs in bulk drug as well as in tablet dosage form.
Key words:- RP-HPLC, Metoprolol succinate, Telmisartan, Simultaneous estimation. |
Filename | Jain Nilesh et al.(102-106).pdf |
Filesize | 271.53 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 11/04/2012 00:00 |
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Last updated on | 12/02/2012 14:49 |
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