Property | Value |
Name | Phytochemical analysis and effect of whole Eclipta alba ethanolic extract on the central nociception and inflammation in rodent models |
Description | Research article:-Biochemistry and Molecular Biology Md. Atiar Rahman1*, Md. Nazim Uddin1, Nazim U. Ahmed2, Md. Rafiqul Islam3 1Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh. 2Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1000, Bangladesh. 3Department of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong-4331, Bangladesh. Abstract:- Aim: The present study was undertaken to investigate the qualitative presence of phytochemicals in Eclipta alba ethanol extract. Antinociceptive and anti-inflammatory mode of action of the extract was also investigated in some rodent models. Materials and methods Phytochemical tests were made by established methods. Antinociceptive effect of the extract was evaluated by acetic acid induced writhing response model, formalin induced licking response model and thermal pain models of Swiss albino mice. The anti-inflammatory effect of the extract was measured volumetrically using the mean increase of hind paw volume of carrageenan induced Wistar albino rats. Data were analyzed by statistical software SPSS using one way ANOVA. Results:- The study confirmed the presence of alkaloids, glycosides, O-glycosides, flavonoids, terpenoids, steroids, tannins, phlobatannins and saponins in the extract. The extract at a dose of 1.0, 1.5 and 2.0 g/kg significantly (P < 0.05) inhibited the acetic acid induced pain. Similar dosages inhibited the formalin induced pain which was higher in the first phase than in the second phase. Thermal pain model showed the maximum possible effect 58.97% at the 120th minute with 2.0 g/kg of extract. The extract significantly reduced the carrageenan induced paw edema by 36.48, 40.65 and 42.41%, respectively at the 4th hour of oral administration. Eclipta alba extract was not found to be toxic in acute toxicity test. Conclusion:- The data of this study suggest that whole Eclipta alba ethanolic extract has excellent central antinociceptive and anti-inflammatory effects and no acute toxicity at least in this experimental condition.
Key words:- Eclipta alba, Antinociceptive, anti-inflammatory, central nociception, rodent model. |
Filename | Rahman Atiar et al.(130-140).pdf |
Filesize | 655 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 12/03/2012 00:00 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 4275 Hits |
Last updated on | 12/04/2012 02:19 |
Homepage | |
CRC Checksum | |
MD5 Checksum |