Property | Value |
Name | Isolation of quercetin- from the leaves of Azadirachta indica and anti-diabetic study of the crude extracts |
Description | Research article:- Applied chemistry Aarti chourasiya* , Anil Upadhayay and R.N. Shukla. P. G. Department of applied chemistry, Samrat Ashok technological Institute,Vidisha (M.P.) India.
Abstract:- Quercetin was isolated from the ethyl acetate soluble fraction of the Methanol extract of the fresh leaves of Azadirachta indica (Family: Meliaceae). The crude extract of hexane, ethyl acetate and butanol soluble fractions of this methanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethyl acetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61μg/ml. Flavonoid "quercetin" was isolated & identified from leaves of Azadirachta indica (Neem). The dried samples were separately soxhlet apparatus & extracted in methanol & then Methanol extract of Neem leaves was subjected to fractionation by column chromatography. The purification of quercetin by repeating TLC. The Rf value of isolated quercetin and standard quercetin was calculated. The purified material was subjected to its IR spectra, & identified as quercetin. This study is also of practical importance because quercetin is an important ingredient of Azadirachta indica.
Key words:- Azadirachta indica, Quercetin, column chromatography, Thin Layer Chromatography (TLC), F.T.I.R, spectrophotometer. |
Filename | Aarti chourasiya ,Anil Upadhayay , R.N. shukla.(179-181).pdf |
Filesize | 310.53 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 12/03/2012 00:00 |
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Maintained by | Editor |
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Last updated on | 12/15/2012 01:53 |
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