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Name | Antibacterial activities of the extracts of the root of Guiera Senegalensis J. F. Gmel |
Description | Research article:- Biochemistry Yagana S. A., Sodipo O.A., Yagana K. and Modu S. Department of Biochemistry, University of Maiduguri – Nigeria.
Abstract:- The methanolic / aqueous, ethylacetate, n-butanol and residual extracts of the root of G. senegalensis were subjected to preliminary phytochemcial screening and in vitro antimicrobial tests. The extracts revealed the presence of flavonoids, tannins, saponins, carbohydrates and cardiac glycosides. Anthraquinones were not detected. The antimicrobial activities of the extracts were assayed by the agar disc diffusion and nutrient broth dilution techniques. Test microorganisms were Streptococcus Pyogene, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Salmonella typhimirium and Klebsiella penumoniae; all the organisms were laboratory isolates. The ethylacetate extract inhibited the growth of Streptococcus pyogene with a mean range of (16.86±0.32 t 20.03±0.55)mm at the concentrations used ranging from (200 – 1000) mg/ml, Staphylococcus with a mean range of (13.16±0.28 to 16.865.51) and Pseudomonas aeruginosa with a mean range of 10.03±14.86±0.32)mm. There was no inhibition by the ethylaceatte extract against Proteus mirabilis, Escherichia coli, Salmonella typhimirium and Klebsiella Penumoniae. The results showed minimum inhibitory concentration (MIC) of 25mg/ml against Streptococcus Pyogene and Staphylococcus aureus while for Pseudomonas aeruginosa was 100mg/ml. The minimum bacteriocidal concentration (MBC) against S. Pyogene and S. aureus was 50mg/ml while that of P. aeruginosa was 100mg/ml. The methanolic / aqueous extract inhibited the growth of S. pyogene with a mean range of (16.09 ± 0.50 to 22.83 ±0.28)mm, S. aureus was inhibited with a mean range of (15.0±0.50 to 21.83±0.28)mm P. aeruginosa was inhibited with a mean range of (9.0±0.50 to 16.0±0.05) mm and P. mirabilis was with a mean range of (12.0±0.50 to 18.0±0.05)mm. There was no inhibition exhibited by the methanolic / water extract against E. Coli, S. typhimirium and K. pneumoniae. The minimum inhibitory concentration (MIC) against S. pyogene was 25mg/ml, for S. aureus was 50mg/ml and that of P. mirabilis and P. aerugonosa were 100mg/ml respectively. The minimum bacteriocidal concentration (MBC) against S. Pyogene and S. aureus were 50mg/dl and that of P. mirabilis was 100mg/dl of P. aeruginosa was 200mg/dl. The n-butanol extract inhibited the growth of S. Pyogene, S. aureus and P. aeruginosa with means ranging from (17.0±0.50 to 18.0±0.50, 18.0±0.50 to 19.0±0.50 and 8.0±0.50 to 17.0±0.50)mm respectively. The minimum inhibitory concentration of S. pyogene and S. aureus was 25mg/ml while that of P. aeruginosa was 100mg/ml. The (MBC) for both S. pyogene and S. aureus was 50mg/ml while that of P. aeruginosa was 200mg/ml. However, even the residual extract inhibited the growth of the test organisms with S. pyogene having mean ranging from (14.66±0.50 to 18.0±0.50)mm, S. aureus having (11.0±0.50 to 16.0±0.50)mm and P. mirabilis with mean ranging from (15.33±0.76 to 17.0±0.50)mm. The results of the (MIC) revealed that S. pyogene and P. mirabilis have (MIC) of 25mg/ml while that of S. aureus was 100mg/ml. The (MBC) for both S. pyogene and P. mirabilis was 50mg/ml while that of S. aureus was 100mg/ml. This study has justified the traditional use of this plant for the treatment of stomach discomfort, diarrhoea, dysentery and as a remedy for wound healing, boils and other infections whose causative agents are some of the organisms used in this study.
Key words:- Minimum inhibitory concentration, G. Senegalensis. |
Filename | Yagana S A. et al..pdf |
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Created On: | 10/05/2012 00:00 |
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Last updated on | 01/05/2013 03:14 |
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