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Name | Characterization of multiple drug resistant uropathogenic Escherichia Coli (UPEC) with special reference to B2-O25B-ST131-CTX-M-15 India. |
Description | Research article:- Microbiology Savita Jadhav1*, Nageswari Gandham1, Arundhuti Paul1, Misra R.N.1, Mani K.R.2. 1Department of Microbiology. Pad. Dr. D. Y. Patil Medical College and Hospital, Pimpri-Pune-18,India 2National Referral Centre Central Research Institute , Kasauli Himachal Pradesh,India. Abstract:- Introduction- Escherichia coli (E. coli) is a remarkable and diverse organism. This normally harmless commensal needs only to acquire a combination of mobile genetic elements to become a highly adapted pathogen capable of causing a range of diseases, from gastroenteritis to extra- intestinal infections of the urinary tract, bloodstream and central nervous system. The worldwide burden of these diseases is staggering, with hundreds of millions of people affected annually. Uropathogenic E. coli (UPEC) cause serious infections in people at risk and have significant environmental prevalence due to contamination by human and animal excreta. In developing countries, UPEC assumes importance in certain dwellings because of poor community /personal hygiene and exposure to contaminated water or soil. Materials and methods- A total of 50 E. coli isolates from urine of patients suffering from UTI and 50 E. coli isolates from the feces of healthy volunteers, were included in the study. The incidence of UTI (due to E. coli) was more in females (70%) than in males (30%). Ability of these E. coli strains to produce the virulence factors viz. production of haemolysin(28%),Cytotoxic necrotizing factor(44%) ,afimbrial adhesion (76%) ,pap(46%), aer(84%) areobactin were seen. E. coli isolates from chronic renal failure were usually bestowed with multiple virulence factors. 60-65% of the E.coli isolates were multidrug resistant (resistant to ampicillin, clotrimoxazole, norfloxacin, tetracycline and gentamicin). ESBL production was demonstrated in 85.77% isolates from UTI and 14.28% were MBL producer.36.66% were ST131-CTX-M-15 MDR UPEC which is markedly high number. Conclusion:- We have standardized and used a rapid simple and inexpensive PCR test that allows the detection of the E.coli O25b-ST131 clone that often produces CTX-M-15enzyme, which may also host other ESBLs.This assay will help to trace the highly resistant and virulent O25b-ST131 clone in the community and hospitals.
Key words:- Uropathogenic E. coli (UPEC), Virulence factors, Multidrug resistance, sequence type B2-O25b-ST131-CTX-M-15. |
Filename | Jadhav Savita et al..pdf |
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Created On: | 10/05/2012 00:00 |
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