Property | Value |
Name | Chemokine, Chemokine receptor, Cytokine, and Apoptosis gene polymorphisms in Tunisian hemodialysed patients with HCV Infection |
Description | Research article:- Leila Ksiaa Cheikhrouhou (MD), Yousr Lakhoua Gorgi (phD), Salwa Jendoubi Ayed (MD), Houda Aouadi (MD) , Imen Sfar (MD), Mouna Makhlouf (MD), Taieb Ben Abdallah (phD), Khaled Ayed (phD). Immunology Research Laboratory of Kidney Transplantation and Immunopathology (Laboratoire de Recherche: LR03SP01). University Tunis El Manar; Charles Nicolle Hospital. Tunisia.
Abstract:- The aim of this study was to investigate the possibility of a significant relationship between the spontaneous clearance or the persistence of HCV infection and chemokines , chemokine receptors, cytokines and apoptosis genes polymorphisms in Tunisian hemodialysed patients. The polymorphisms of genes: CCR5 Δ32, CCR5 (-59029) A/G, CCR2 (64Ile), and MCP-1(-2518) A/G, (-889) IL-1α, (-511) and (+3954) IL-1β, IL-1-Ra, IL-18 (-137) and (-607), IL-12p40 (-1188) and Apo1/Fas (-670) were determined by PCR-RFLP, PCR-SSP and PCR-VNTR, at healthy blood donors (100 for cytokines and apo/fas gene polymorphisms and 170 for chemokine gene polymorphisms) and 100 hemodialysed patients infected by the HCV. Patients were classified in two groups: G1 included 76 active chronic hepatitis patients (positive RNA-HCV) and G2 included 24 having eliminated spontaneously virus (negative RNA-HCV). The univariate analyze of the genotypes and alleles frequencies of the cytokines polymorphisms studied does not reveal any positive or negative association statistically significant with the outcome of the HCV infection. Nevertheless, the frequency of genotype association [-37GC/-607CA] IL-18 is statistically higher among G2 patients (41,7%) compared to that at G1 hemodialysed (15,8%) (p=0,008, OR: 0.26, 95%CI: [0.10-0.73]). We found also, a significant increased frequency of AA genotype of Apo1/Fas gene in G2 patients (41,6%) than in G1 (17,5%) (p=0,026, OR=3,49, 95% CI [1,13-10,69]). Adjustment for known covariates factors (age, gender and genotypes) confirmed these univariate findings and revealed that the genotype association GC-CA of (-137and-607) IL-18 gene and AA genotype of Apo1/Fas gene were associated to the clearance of HCV (p=0.041 and p=0.017 respectively). Our results showed also a statistically increased frequencies of the CCR2 (64Ile) and the (-59029) CCR5 A alleles in total patients HCV infected (22,1% and 35,9%) and in G1 (24,3% and 40,6%) compared to controls (14,4% and 20%)[p=0.0086, p=0.03 and p=0.04, p=0.017 respectively]. Also, we observe a lower frequency of the MCP-1 G allele and a greater frequency of the CCR5 Δ32 variant in G2 (15,2% and 6,5%) compared to G1(22,6% and 1,4%)[p>0.05], but adjustment for known covariates factors didn’t confirmed these univariate findings. In conclusion, our study suggests a possible role of some of the chemokine and cytokine and apo/fas polymorphisms studied in the outcome of HCV infection in Tunisian population. These results should be further investigated by large population-based studies.
Key words:- Hepatitis C virus, Spontaneous clearance, Chemokines, Chemokines Receptors genes polymorphisms, cytokines, apo/fas polymorphisms.
Copyright © 2013 Leila K Cheikhrouho et al., This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Filename | Leila K Cheikhrouho et al.(432-450).pdf |
Filesize | 673.45 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 01/04/2013 00:00 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 2344 Hits |
Last updated on | 02/19/2013 03:03 |
Homepage | |
CRC Checksum | |
MD5 Checksum |