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Name | Evaluation of semiquantitative culture method in the diagnosis of central venous intravascular catheter related infections |
Description | Original article:- Microbiology Nirma.S.Amin1,Nilica Devi Sh.1 & Sevitha Bhat2* 1M Sc, Medical Microbiology, 2Associate Professor ,Department of Microbiology ,Kasturba Medical College, Mangalore- 575001.,India.
Abstract:- Background & objectives: The use of intravascular devices frequently is complicated by a variety of local or systemic infectious complications, including septic thrombophlebitis, endocarditis, bloodstream infection (BSI). Catheter- related infection is defined according to catheter-tip colonization, catheter related local infection (CRLI) and catheter related blood stream infection (CRBSI). The semiquantitative culture technique is useful in the diagnosis of bacteremia associated with central venous catheters. The present study was undertaken to evaluate the usefulness of semiquantitative culture of catheter tips of Central venous catheters. Materials and Methods: The cross sectional study was conducted in the Department of Microbiology, KMC, Mangalore. This study included 63 catheter tips and blood culture samples from patients with suspected intravascular catheter related infection. Antibiotic susceptibility testing was performed by Modified Kirby-Bauer disc diffusion method. Results: The overall tip colonization was 32 (50.79%) had growth >15 CFU and 31 (49.2%) had no growth. Out of the 32, definite catheter associated bacteremia was 5(7.93%), catheter associated infection was 18(28.57%), probable catheter associated bacteremia was 9(14.28%). The organisms isolated were Staphylococcus aureus, Enterococcus spp, Streptococcus pneumoniae, E.coli, Klebsiella spp, Citrobacter spp, Acinetobacter spp, Candida albicans & C. tropicalis. Conclusion: There is significant lack of specificity (a high number of false-positive results) of the Semi quantitative technique for the diagnosis of CRBSI , while its sensitivity is probably satisfactory. Key words:- Catheter related sepsis , HAI , Device associated infection.
References:- 1.Singh S, Pandya Y, Patel R, Paliwal M, Wilson A, Trivedi S.et al. Surveillance of Device-associated Infection at a teaching hospital in Rural Gujrat- India. Indian J Med Microbiol . Oct-Dec2010; 28, 4:342-7. 2.Forbes A B, Sahm F D, Weissfeld S A, Bloodstream Infections, Bailey and Scott’s Diagnostic Microbiology, twelfth edition, Mosby Elsevier,2007. 3.Andremont A.,Paulet R.,Nitenberg G., Hill C. Value of Semi quantitative cultures drawn through catheter hubs for estimating the risk of catheter tip colonization in cancer patients. J Clin Microbiol 1988; 26: 2297-9. 4.Linares, J., A. Sitges-Serra, J. Garau, J.L. Perez, and R. Martin. Pathogenesis of catheter sepsis: A Prospective Study with Quantitative and Semi Quantitative Cultures Of Catheter Hub and Segments. J Clin Microbiol 1985; 21: 357-360. 5.Sherertz RJ, Heard SO, Raad I I. Diagnosis of Triple Lumen Catheter Infection: Comparison of Roll Plate, Sonication, and Flushing Methodologies. J Clin Microbiol 1997; 35: 641-6. 6.Beekmann S.E, Henderson D.K, Infections caused by Percutaneous Intravascular devices; Mendell, Douglas and Bennett’s Principles and Practice of infectious diseases, Sixth edition, 2: 3347-58. 7.Harsha V.Patil, Virendra C. Patil, M.N. Ramteerthkar, R.D. Kulkarni. Central venous catheter-related bloodstream infections in the Intensive Care Unit. IJCCM Oct-Dec 2011; 15:213-20. 8.Maki, D. G., C. E., Weise, and H. W. Sarafin. A Semiquantitative Culture Method For Identifying Intravenous Catheter-Related Infections. N. Engl. J. Med 1977; 296 : 1305-9. 9.Collignon J P, Soni N, Pearson Y I, Woods P W, Munaro R, Sorrell C T. Is semiquantitative Culture of Central Vein catheter tip useful in the diagnosis of catheter – associated bacteremia? J Clin Microbiol 1986; 24: 532-5. 10.Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests; Twentieth informational supplement. CLSI document.M 100-S 20 ,Vol 30 ,No. 1 . Jan 2010.pg 48-51.
Competent interest: - The authors declare that they have no competing interests.
Source of funding: - None.
Copyright © 2013 Savitha Bhat. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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