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Name | Comparison of collagen IV expression in well-differentiated squamous cell carcinomas by immunohistochemistry and mRNA analysis |
Description | Original article:-Oral and Maxillofacial Pathology Lalita Jayaram Thambiah1* & Satish Kumaran P2 1Professor of Oral and Maxillofacial Pathology, Consultant Oral and Maxillofacial Pathologist,Annasamy Mudaliar General Hospital, Fraser town, Bangalore,India. 2Consultant Oral and Maxillofacial Surgeon,Annasamy Mudaliar General Hospital,Fraser town, Bangalore,India. Abstract:- Malignant tumours which start in one part of the body tend to invade organs not directly connected with that part and develop tumours in them. Destruction of the basement membrane is the first step in tumour invasion and metastasis. The expression of collagen IV protein and the expression of mRNA of collagen IV were studied to ascertain if they correlate. Malignant tissues have a high molecular mass which specifically degrades collagen IV. Many tumourigenic cells synthesize less collagen and larger quantity of proteases including collageneases. It results in the cells becoming less dependent on extracellular matrix for growth. These factors increase their ability to invade.
Keywords:-Well-differentiated squamous cell carcinoma, metastasis, collagen IV, mRNA expression level.
References:- 1.Daniela Spano et al. “Molecular Networks that Regulate Cancer Metastasis.” Seminars in Cancer Biology. 22 (2012) 234-49. 2.Massoumeh Zargaran et al. “Immunohistochemical Evaluation of Type IV Collagen and Laminin-332 γ2 Chain Expression in Well-differentiated Oral Squamous Cell Carcinoma and Oral Verrucous Carcinoma: a New Recommended Cut-off.” Journal of Oral Pathology & Medicine (2011) 40:167-73. 3.Michio Tsuda, Yumi Yamagishi and Tsunehiko Katsunuma. “High Molecular Mass Type IV Collagen-specific Metalloprotease from Human Carcinoma Tissue.” Federation of European Biochemical Societies Vol. 232, No.1, 1988: 140-4. 4.Real-time Polymerase chain reaction. 5.Kenneth J.Livak and Thomas D. Schmittgen, “Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-∆∆CT” methods, 2001; 25, 402-8. 6.Timpl, R., Wiedmann, H., von Delden, V., Furthmayr, H and Kuhn, K.: “A Network Model for the Organization of Type IV Collagen Molecules in Basement Membranes.” Eur.J.Biochem 1981; 120:203-11. 7.Hynda K. Kleinman, Robert J. Klebe and George R. Martin. “Role of Collagenous Matrices in Adhesion and Growth of Cells.” The Journal of Cell Biology 1981;88 (March): 473-85.
Copyright © 2013 Lalita Jayaram Thambiah & Satish Kumaran P. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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