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Name | Clinical significance of the effect of Gongronema Latifolium leaf extract on some serum enzymes in diabetic rats |
Description | Original article:- Biochemistry E. E. Edet1, E. David-Oku1* M.I. Akpanabiatu2, G. O. Igile1, B. Mgbeje1, G. E. Egbung1 & F.E.Uboh1 1Department of Biochemistry, University of Calabar, P.M.B. 1115, Calabar, Nigeria. 2Department of Biochemistry, University of Uyo, P.M.B. 1017, Uyo, Nigeria.
Abstract:- The effect of Gongronema latifolium crude leaf extract (GL) on serum enzymes was assessed in male diabetic rats. The rats were gavaged with 200, 300 and 400 mgkg-1 b.wt. of GL day-1 for two weeks. Serum was investigated biochemically for AST, ALT, ALP, GGT and LDH activities. Serum ALT, ALP and LDH activities increased significantly (p< 0.001) in alloxan-induced diabetic rats treated with GL when compared to non-diabetic rats. In diabetic rats, serum GGT activity increased significantly (p< 0.05) in the treated animals, whereas serum LDH activity decreased significantly (p< 0.01) when compared to their controls. In diabetic rats also, AST: ALT ratios were higher in the treated animals when compared to the control and showed non-dose dependent decreases in serum AST, ALT, LDH and GGT activities with increased dosage of GL. In non-diabetic rats, serum ALP and LDH activities decreased significantly (p< 0.05) in the treated animals when compared to the control. There were also non-dose dependent decreases in serum AST, ALT, ALP, GGT and LDH activities with increased dosage of GL. AST: ALT ratio was high in treated animals when compared to the control and showed a non-dose dependent increase with increased dosage of GL. Moreover the AST: ALT ratio of test groups and controls of both diabetic and non-diabetic rats were higher than unity or one. The high values of AST: ALT ratio in the serum provided additional evidence for the incidence of myocardial infarction in this study. The actions of the water soluble fraction of the ethanol extract in diabetic and non-diabetic wistar rats suggest different mechanism of action of GL leaf extract.
Key words:- Alloxan-induced diabetes, Gongronema latifolium, Serum enzymes, Myocardial infarction.
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