Property | Value |
Name | The clinical utility of typhidot in the diagnosis of typhoid fever |
Description | Original article:-Microbiology B. Appalaraju1, Anila A.Mathews2* & Priya P3 1Professor and Head, 2Associate professor, 3MBBS Student, Department of microbiology, PSGIMS&R, Coimbatore, India.
Abstract :- Background: Typhoid Fever is a severe systemic infection caused by Salmonella typhi. It is endemic in many developing countries, particularly in South East Asia. Clinical diagnosis is often unreliable and isolation of S. typhi through blood culture is still used as the gold standard, although, at its best, the blood culture yield is only 70-75% in cases of typhoid fever. Objective: To evaluate Typhidot test and to compare the results of typhidot test with Blood culture and Widal test and to correlate it clinically Materials and method: A pilot study was carried out on the blood samples that came to our lab during the two months of study with history of typhoid fever or Pyrexia of unknown origin. The Typhidot is a dot ELISA which detects IgM and IgG antibodies against Salmonella typhi. Results: Of the 81 blood samples ,45 were positive for Salmonella typhi infection, 4 were positive for Salmonella paratyphi A , 32 were negative for typhoid fever. Sensitivity and specificity of typhidot for detection of Salmonella typhi infection was 100%and 63% respectively. Sensitivity and specificity of widal test in comparison with blood culture was 82%and 65% respectively Discussion and conclusion: Blood culture is still the gold standard for diagnosis of typhoid fever. Typhidot was found to have a better sensitivity, specificity and positive predictive value than Widal test. The results can then be confirmed by blood culture report. This test can be used in areas where facility for blood culture is not available. Since it is an inexpensive test, takes less time in producing results and has excellent sensitivity, it can be used in all labs.
Key Words: Typhidot, typhoid.
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Copyright © 2013 B. Appalaraju, Anila A.Mathews & Priya P. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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