Property | Value |
Name | Chronic administration of Fluoxetine ameliorates depression: Enhanced BDNF and its receptor TrkB expressions with down stream signalling cascades ERK1/2 and Akt pathways |
Description | Original article:- Physiology Ritabrata Banerjee1, Anup Kumar Ghosh2, Balaram Ghosh3 & Amal Chandra Mondal1* 1Senior Research Fellow (SERB Project), 1*Assistant Professor in Physiology, Raja Peary Mohan College, Uttarpara, Hooghly, University of Calcutta, West Bengal-712258,India. 2Assistant Professor in Instrumentation Science, Jadavpur University, Kolkata, West Bengal-700032, India. 3Assistant Professor in Pharmacology, Calcutta Medical College and Hospital, Kolkata, West Bengal-700072, India.
Abstract:- Objectives: Stress-induced helplessness in rodents constitutes a well-defined model to investigate neurobiological mechanism of depression. In the present investigation, we investigated the correlation between stress induced helplessness and BDNF, its receptor TrkB with two of its principal downstream signaling molecule ERK1, 2 and Akt. Background: Brain-derived neurotrophic factor (BDNF) regulates many physiological functions in the brain, alteration of which has been well associated with the pathogenesis of depression. Materials and Methods: BDNF level was measured by Sandwich ELISA and its cognate receptor TrkB and downstream molecules ERK 1, 2, Akt were assayed by Western blot. Results: Chronic stressed rats exhibited down regulation of BDNF and TrkB along with ERK1, 2 and Akt. This parallels with the decreased escape behavior. The antidepressant drug Fluoxetine hydrochloride (FLX) treated rats exhibited significant increase in escape deficit in stress induced Learned helplessness (LH). This was correlated with the restoration of BDNF levels, its cognate receptor TrkB and expression of its down stream signaling molecules ERK1, 2 and Akt in hippocampus of rat brain. Conclusion: This supports the notion that pharmacological restoration of BDNF and its receptor TrkB may be of therapeutic value for the treatment of depression.
Key Words:-Depression, Neurotrophin, BDNF, TrkB, ERK 1, 2, Akt, FLX.
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