Property | Value |
Name | Anthelmintic activity of Balanites aegyptiaca against Haemoncus contortus in Goats |
Description | Research article:- A.M. Osman Intisar 1,Ibtisam Goreish2,Sania A. Shaddad3*, Tigani H. Elamin4 & I.B. Eltayeb3
Affiliation:- 1Department of Pharmacology, College of Veterinary Medicine, University of Bahri, Sudan. 2Department of Parasitology,Central Veterinary Research laboratories (Soba), Khartoum, Sudan. *3Department of pharmacology, Faculty of Pharmacy, University of Khartoum, Post box 321, Sudan. 4Department of pharmacology Faculty of Veterinary Medicine,University of Khartoum, Post box 321, Sudan.
Abstract:- Introduction: The development of anthelmintic resistance and the high cost of anthelmintic drug led to evaluation of medicinal plants as alternative source of anthelmintic. The aim of current study, experiments were conducted to determine the possible anthelmintic effects of aqueous and methanolic extracts of Balanites aegyptiaca kernel, in vitro using live Haemoncus contortus and in vivo using experimentally induced Haemoncus contortus infection in Nubian goats. Methods: Methanolic extracts of the plants were performed according to the method of Harborne (1984). Aqueous extracts of the plants were performed according to the method of Fenado et al. (1989). In vitro and in vivo anthelmintic activity of both crude aqueous and methanolic extracts of Balanites aegyptiaca were studies against adult H.contortus as evident from mortality rate of the worms. 31 goats (6-8 month old) healthy male Nubian goats weighing 10-12kg were used in this experiment divided into 4 groups. Group 1: Non infected goats served as negative control. Group 2: Infected goats with Haemoncus contortus received no treatment served as positive control. Group 3: Infected with Haemoncus contortus divided into 2 sub groups each of five animals treated orally with two doses of aqueous extract of Balanites aegyptiaca ( 100&400mg /kg). Group 4: Goats infected with Haemoncus contortus divided into 3 sub groups each of five animals treated orally with three doses of methanolic extract of Balanites aegyptiaca ( 100&400&800mg /kg). Faecal samples from each animal were collected in the morning starting from day zero pretreatment and at day 7, 14, 21 post treatment and examined for the presence of worm eggs by flotation technique (MAFF, 1979). The eggs count was performed by Mc Master Method (Soulsby, 1982). the numbers of adult or mature worms H. contortus in the aliquots were counted. Results: The in vitro study revealed significant anthelmintic effects ( p≤0.05 )for both crude aqueous extract(CAE) and crude methanolic extract (CME) of Balanites aegyptiaca kernel on live Haemoncus contortus as evident from their mortality or temporary paralysis. For in vivo studies, the CME and CAE of Balanites aegyptiaca revealed dose dependent anthelmintic effects. The three doses (100,400,800 mg/kg) of CME of B. aegyptiaca showed 20%, 74.7% and 79.2% reduction in eggs count per gram of faeces (EPG) on day 21post treatments respectively. However, the dose of 100mg/kg CAE of Balanites aegyptiaca showed no significant effect while, the dose of 400mg/kg revealed 61.4% reduction in EPG on day 21post treatments.
Key Words:- In vivo, In vitro, Anthelmintic, Balanites aegyptiaca, Haemoncus contortus, Goats.
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