Property | Value |
Name | Bacteriological Study of Urinary Tract Infections with special reference to Extended Spectrum Beta Lactamase producing Escherichia coli and Klebsiella pneumoniae |
Description | Research article:- Dr.Girishbabu R J1*, Dr.Prakash R1 & Dr.Prashanth H V2
Affiliation:- 1 Assistant Professor, 2 Professor ,Department of Microbiology, Sri Siddhartha Medical College, Tumkur- 572107, Karnataka, India.
Abstract: Background: Urinary tract infections are among the most common bacterial infections that lead patients to seek medical care. The incidence of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae have been steadily increasing over the past few years resulting in limitation of therapeutic options. Aims & Objectives: The aim of the study was to isolate, identify and to establish the antimicrobial susceptibility pattern of the pathogens responsible for urinary tract infections and also to determine the extended spectrum beta lactamase production of Escherichia coli and Klebsiella pneumoniae. Settings and design: Hospital based Prospective study. Materials & Methods: The study includes 3060 clinically suspected cases of urinary tract infection over a period of one year. Isolates were identified by conventional methods. Isolated Escherichia coli and Klebsiella pneumoniae which showed resistance to cefotaxime and ceftazidime were tested for extended spectrum beta lactamase production by phenotypic confirmatory test as proposed by the Clinical and Laboratory Standards Institute document. Statistical Analysis: The results were analyzed using mean, median and Chi-square (χ2) test. Results: Of the 3060 urine samples processed 990 (32.35%) samples yielded various bacterial isolates. Extended spectrum beta lactamase production was observed in 174 (38%) of Escherichia coli isolates and in 102 (42%) of Klebsiella pneumoniae isolates. Conclusion: Our results showed Escherichia coli as predominant organism, followed by Klebsiella pneumoniae causing urinary tract infection. Klebsiella pneumoniae were found to be more extended spectrum beta lactamase producer when compared to Escherichia coli.
Key Words:- Escherichia coli, Extended Spectrum Beta Lactamase, Klebsiella pneumoniae, Urinary tract infection.
References:- 1.Noor N, Ajaz M., Rasool SA., Pirzada ZA. Urinary tract infections associated with multidrug resistant enteric bacilli, characterization and genetical studies. Pak J Pharm Sci. 2004;17: 115-123 2.Ronald AR, Pattulo MS. The natural history of urinary infection in adults. Medical clinics of North America 1991; 75: 299-312. 3.Wilson ML, Gaido L. Laboratory diagnosis of urinary tract infections in adult patients. Clin Infect Dis. 2004; 38: 1150–1158. 4.Sobel JD, Kaye D. Urinary tract infections. In: Mandel GL, Bennett JE, Dolin R, editors. Mandell, Douglas, and Bennett’s principles and practice of infectious diseases. 5th ed. Newyork: Churchill Livingstone; 2000. p. 773-805. 5.Newell A, Riley P, Rogers M. Resistance patterns ofurinary tract infections diagnosed in a genitourinary medicine clinic. Int J STD AIDS 2000; 11: 499-500. 6.Hryniewicz K, Szczypa K, Sulikowska A, Jankowski K, Betlejewska K, Hryniewicz W. Antibiotic susceptibility of bacterial strains isolated from urinary tract infections in Poland. J Antimicrob Chemother 2001; 47: 773-80. 7.Cao V, Lambert T, Nhu DQ, Loan HK, Hoang NK, Arlet G, Courvalin P. Distribution of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae in Vietnam. Antimicrob Agents Chemother 2002; 46: 3739-43. 8.Bradford PA. Extended-spectrum beta-lactamase in the 21st century: characterization, epidemiology, and detection of this important resistance threat, Clin Microbiol Rev 2001; 14: 933-51. 9.Cormican M, Morris D, Corbett-Feeney G, Flynn J. Extended-spectrum beta-lactamase production and fluoroquinolone resistance in pathogens associated with community-acquired urinary tract infection. Diagn Microbiol Infect Dis 1998; 32: 317-319. 10.Bush K, Jacoby GA (2010). An updated functional classification of β-lactamases. Antimicrob. Agents Chemother. 54(3): 969-76. 11.Babypadmini S, Appalaraju B. Extended spectrum beta lactamases in urinary isolates of Escherichia coli and Klebsiella pneumoniae- prevalence and susceptibility pattern in a tertiary care hospital. Indian J Med Microbiol 2004; 22(3): 172-4. 12.Bou G, Cartelle M, Tomas M, Canle D, Molina F, Moure R, Eiros JM, Guerrero A. Identification and broad dissemination of the CTX-M-14 β-lactamase in different Escherichia coli strains in the Northwest area of Spain. J Clin Microbiol 2002; 40: 4030-6. 13.Koneman EW, Allen SD, Janda WM, Winn WC, Schreckenberger PC, Procop GW, et al, editors. Introduction to microbiology- part II: urinary tract infections. In: Koneman’s color atlas and textbook of diagnostic microbiology. 6th ed. Philadelphia: Lippincott Williams and Wilkins; 2006. p. 82-87. 14.Forbes BA, Sahm DF, Weissfeld AS, editors. Infections of the urinary tract In: Bailey & Scotts diagnostic microbiology. 11th ed. St.Louis: Mosby; 2002. p. 927-938. 15.Collee JG, Duguid JP, Fraser AG, Marmion BP, Simmons A. Laboratory strategy in the diagnosis of infective syndromes. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney, practical medical microbiology. 14th ed. Edinburgh: Churchill Livingstone; 1996. p. 84-90. 16.Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 17th informational supplement. CLSI/NCCLS document M100-S17. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania; 2007; 27(1): 1-177. 17.Water G, Harrison B, and Kunin G. Urinary tract infection. N. Eng Med. J 1996; 248-50. 18.Urinary Tract Infection. Adam healthcare center. [Online]. 2007 [cited 2007 Sept 16]; Available from: URL:http://www.about .com 19.Das RN, Chandrashekhar TS, Joshi HS, Gurung M, Shrestha N, Shivananda PG. Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in Western Nepal. Singapore Med J 2006; 47(4): 281-5. 20.Mohanty S, Kapil A, Das BK, Dhawan B. Antimicrobial resistance profile of nosocomial uropathogens in a tertiary care hospital. Indian J Med Sci 2003; 57: 148-54. 21.Anbumani N, Mallika M. Antibiotic resistance pattern in uropathogens in a tertiary care hospital. Indian J Practising Doctor 2007; 4(1): 204-7. 22.Shobha KL, Rao GS, Rao S, Sreeja CK. Prevalence of extended spectrum beta-lactamases in urinary isolates of Escherichia coli, Klebsiella and Citrobacter species and their antimicrobial susceptibility pattern in a tertiary care hospital. Indian J Practising Doctor 2007; 3(6): 1-2. 23.Akram M, Shahid M, Khan AU. Etiology and antibiotic resistance patterns of community-acquired urinary tract infections in JNMC hospital Aligarh, India. Ann Clin Microb Antimicrobials 2007; 6: 4. 24.Samaha-Kfoury JN, Araj GF. Recent developments in β-lactamases and extended spectrum β-lactamases. BMJ 2003; 327: 1209-13.
Copyright © 2013 Girishbabu R J, Prakash R & Prashanth H V. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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