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Review article:- Piplani S1, * Lalit M2, Arora AK3.
1.Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, Punjab,India. 2.Chintpurni Medical College,Bungal, Pathankot, Punjab, India.
3.Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, Punjab, India.
Abstract-: Glucagonomas an uncommon clinicopathological entity originate from the alpha-2 cells of the pancreas The syndrome goes by the acronym 4D syndrome, which stands for dermatosis, diarrohea, deep vein thrombosis and depression. Necrolytic migratory erythema (NME) is the most characteristic clinical sign of this pathology. It is associated with hyperglucagonemia, diabetes mellitus, hypoaminoacidemia, cheilosis, normochromic, normocytic anemia, and weight loss. NME features a characteristic skin eruption of red patches with irregular borders, intact and ruptured vesicles, and crust formation.The tumor cells are occasionally organized in nests and strands and appear strongly glucagon-positive on immunohistochemical staining. The correct recognition of NME is very important, because it may allow early detection either of glucagonoma or of extrapancreatic, glucagon-secreting tumors. Glucagonoma is a slow growing tumor and good recovery is possible after surgical resection.
Key words:- Glucagonoma, Hyperglucagonemia, Necrolytic migratory erythema (NME), Diabetes mellitus.
Research article:- * P. Shetti1 , A. Venkatachalam.1 1.Department of Chemistry, Bhavan’s College, Andheri (West), Mumbai – 400058, Maharashtra, India
Abstract:- Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorpromazine HCl is antipsychotic drugs and used for symptomatic treatment of psychotic ailment. We present a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method developed and validated for the quantification of Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorpromazine HCl in Human Plasma. Ornidazole was used as the internal standard. The simple and cost effective Liquid-Liquid Extraction method is applied for the extraction of all three drug and Internal standard from Plasma. A rapid isocratic separation is achieved by a short HYPERSIL, Hypurity C18 (50x4.6)mm 5µ (Thermo, USA) column using mobile phase of Acetonitrile and Ammonium acetate buffer (70:30 v/v, (%) ) at flow rate of 0.4 ml/min. The run time is 2 minutes suggests high throughput of the proposed method. . The compound was ionized in the positive electro spray ionization (ESI) mode of the mass spectrometer. Detection of mass was performed in multiple reactions monitoring (MRM) mode; Trihexyphenidyl m/z 302.2-►98.1, Trifluoperazine m/z 408.1-►113.1, Chlorpromazine m/z 319.1-►86.1 and Ornidazole 220.1-►128.1. The response for all the three drugs found to be linear over the range of 0.1-100 ng/ml. The lower limit of quantification of the LC-MS/MS method was 0.1ng/ml. The intra-and inter-day precisions were below 10.0% and the accuracy was between + 5.0% and - 5.0%. This validated LC-MS/MS is rapid, sensitive, specific and cost-effective method for determining Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorporomazine HCl in human plasma samples.
Key words :- Trihexyphenidyl HCl, Trifluoperazine HCl and Chlorpromazine HCl, LC-MS/MS study, Bioequivalence, Development and validation.
Health and Safety Survey:- *Javed Akhter, PhD, Sameera Al Johani, MD, Loulwah Hammad, BSc, Khalid Al Zahrani, MSc.
*Director, Quality Management and Laboratory Safety,Pathology and Laboratory Medicine, Mail Code 1122,P.O. Box 22490,King Abdulaziz Medical City,Riyadh 11426,Saudi Arabia.
Abstract:- A Health and Safety survey was conducted among Laboratory workers in the Pathology Laboratory at King Abdulaziz Medical City, Riyadh, Saudi Arabia. Analysis of 154 completed questionnaires showed that the majority of respondents were female (61%) with most participants under the age of 30 years (49%). 88% of respondents regularly worked with blood and blood products. It was found that 14% had suffered a needle stick injury and 8% had received splashes to the eyes or mouth; 2% had not reported these exposures. Full compliance of wearing gloves at all times when working with blood and blood products was 92%. Only 61% of respondents washed their hands after removing gloves. There was also a low compliance of using goggles/face shields when handling body fluids outside of a biological safety cabinet (17%). Reasons given were lack of availability (30%), not thought necessary (18%) and interfered with job (8%). Total compliance for safe disposal of sharps in a sharps container was 92%. This first report in Saudi Arabia identified several deficiencies and strategies for improvement. These results are essential in formulating effective health and safety measures and provide relevant and focused education to reduce occupational related hazards and injuries.
Keywords:- Occupational hazards, laboratory workers, laboratory hazards.
Research article:- Shenoy Revathi P, MSc, PhD 1 , * Bakkannavar Shankar M, MD, DCL 2, Vidya Monnappa, MD, Diplomate NB3, Bhat Akshay V,MSc 4, Mukesh Kumar, MSc,4 Nayak Vinod C, MD 5, Pradeep Kumar G, MD, Dip.Cr.L 6.
1.Assistant Professor, Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, India.
2.Assistant Professor, Department of Forensic Medicine and Toxicology, Kasturba Medical College, Manipal University, Manipal,India.
3.Associate Professor, Department of Pathology, Kasturba Medical College, Manipal University, Manipal, India.
4.Postgraduate, Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, India.
5.Associate Professor, Department of Forensic Medicine and Toxicology, Kasturba Medical College, Manipal University, Manipal, India.
6.Professor & Head, Department of Forensic Medicine and Toxicology, Kasturba Medical College, Manipal University, Manipal, India.
Abstract:- Myocardial infarction is the most common cause of sudden death. However the identification of myocardial infarcts at necropsy is difficult. Various methods are being used to detect it. It has been shown that the use of dyes such as nitroblue tetrazolium and 2,3,5 triphenyl tetrazolium chloride (TTC) that identify the dehydrogenase deficient infracted myocardium are largely used. But the studies have been conducted largely in animal models using these dyes. Establishing the utility of these enzyme histochemical tests at autopsy on human cadavers is the need of the hour. We studied the fourty hearts of sudden death cases staining them with TTC and thereafter confirming with histology to know the efficacy of TTC staining. Our results showed that this histochemical test is a reliable method of investigation in sudden death cases.
Key words:- Myocardial infarction, 2,3,5 triphenyl tetrazolium chloride, histochemical tests.
Review article:- *Swapnil L. Patil , Madhavi A. Shivnikar
Pharamceutics department,Padm.Dr.D.Y Patil College of Pharmacy, Akurdi, Pune, Maharastra ,India ,411018.
Abstract:- The oral route remains the perfect route for the administration of therapeutic agents because of the low cost of therapy and ease of administration lead to high levels of patient compliance. Fast disintegrating tablets have been formulated for pediatric, geriatric, and bedridden patients and for active patients who are busy and traveling and may not have access to water. Fast disintegrating tablets are those when put on tongue, disintegrates instantaneously, releasing the drug, which disperses or dissolves in the saliva. Mouth dissolve tablets are also known as orodispersible, quick dissolve, fast melt, fast dissolve, fast disintegrate, rapid-dissolve, rapimelts or orally dissolve tablets. European pharmacopoeia recently adopted the term “Oro-dispersible tablet” as a tablet to be placed in mouth where it disappears rapidly before swallowing. Fast disintegrating tablets have received ever-increasing demand during the last decade and the field has become a rapidly growing area in the pharmaceutical industry.
Keywords:- Fast disintegrating tablets, Lyophilization, Direct compression