DocumentsDate added
Research article:-
P.V.Kamala Kumari*, G.Girija Sankar, T.Prabhakar
Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
Abstract:- Aim: Optimization of different process parameters for L-asparaginase production by Streptomyces griseoluteus WS3/1 and its validation using response surface methodology was carried out during the present study. Methods and results: Four medium factors were screened by conventional methods and subsequent optimization process using Central composite design (CCD) was performed to find out the optimium values of the selected parameters. Dextrose, L-asparagine, pH and inoculum level were found to be the best medium factors for the optimization of L-asparaginase production and central composite design experiments indicated the optimal values of dextrose, L-asparagine, pH, inoculum level were 0.45 % w/v, 1.162 % w/v, 7.4 and 10.36 % v/v respectively. Conclusions: The combination of conventional method with central composite design (CCD) was effective and reliable in selecting the statistically significant factors and determining the optimal levels of those factors in fermentation medium. This work demonstrates the usefulness of CCD for determining the optimum conditions for maximizing L-asparaginase production. Significance and impact of the study: The combined optimization method described here is the effective for screening medium factors as well as determining their optimum levels for the production of L-asparaginase by Streptomyces griseoluteus WS3/1.
Key words :-S.griseoluteus WS3/1, L-asparaginase, CCD.
Research article:-
* OC Adekunle, OM Olatunji
*Department of Medical Microbiology & Parasitology Ladoke Akintola University of Technology Osogbo, Nigeria. P.O. Box 14066, U.I. Ibadan, Oyo State.
Abstract:-The study was carried out in Osogbo among hospitalized patients in order to determine the incidence of staphylococcus aureus and the antibiotic sensitivity patterns of organism. Specimen were isolated from the nasal cavities of hospitalized patients between ages 0 to 75years. One hundred and five samples were analyzed. Antibiotic susceptibility tests were performed. All the nasal swab samples were positive for staphylococcus species. The age group 16-31 years had highest carriage rates (35.2%). Forty six (46) were staphylococcus aureus while 59 were other staphycoccus species. All staphylococcus species were sensitive to pefloxacin. The isolation of staphycoccus species in all patients showed high endemicity of the organism in hospital environment. Also, pefloxacin can be used as drug of choice in the environment.
Key words :- Staphylococcus aureus, nasal cavity, hospitalized patients, antibiotic.
Research article:-
*K. Sowparthani 1, G.Kathiravan 2.
*1 Department of Microbiology, Apollo Arts and Science College, Chennai.
2 Department of Biotechnology, VELS University, Pallavaram, Chennai.
Abstract:- Phyllanthus amarus (Schum & Thonn) is predominantly one of the herbs in waste lands thoroughout the plains of India. The leaves are used in traditional medicine. In the present investigation, the isolation and identification of endophytic fungi of Phyllanthus amarus was carried out. Ethyl acetate extract from the midrib and lamina of Phyllanthus amarus were investigated for their antibacterial activity. In the present investigation, the antibacterial screening of ethylacetate extract endophytic fungi from Phyllanthus amarus was carried out. The crude and column purified culture extract of Gleosporium sp. were active against Gram positive (Staphylococcus aureus, Streptococcus pneumoniae) and Gram negative bacteria (Salmonella typhi, E. coli ) pathogenic bacterial strains.
Key words: Keywords:- Phyllantus amarus, Gleosporium sp., endophytic fungi, antibacterial activity.
Research article:-
* Jahir Alam Khan1, Vivek Chaurasia 2 1. R&D division, MRD LifeSciences (P) Ltd., Lucknow, India. 2. Institute of Biosciences & Biotechnology, CSJM University, Kanpur, India.
Abstract:- In the present study, antibacterial activity of cold aqueous, hot aqueous, methanolic, ethanolic, ethyl acetate, and acetone extracts of leaf, stem, bark, ripe and unripe fruit of Psidium guajava were screened against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa using Agar well diffusion method. All the extracts showed significant antibacterial properties. Cold aqueous extract of ripe fruit was found to be the most effective followed by acetone extract of leaf, hot aqueous extract of stem, ethanolic extract of unripe fruit and acetone extract of bark.
Key words:- Psidium guajava, herbal antibiotics, pathogenic, antibacterial properties.
Research article:- *Surendra Kr. Sharma, N. Goyal.
* M. Pharm, Ph. D, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India.
M. Pharm, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India.
Abstract:-In vitro antioxidant activity of methanolic and aqueous root extracts of Pluchea lanceolata was determined by DPPH free radical scavenging assay and hydrogen peroxide scavenging activity. The extract revealed marked activity as a radical scavenger in a study indicating that extracts have ability to donate hydrogen. The absorption is stoichiometric in respect to the number of electrons taken up. Concentration of 0.1 mg/ml of methanolic extract (PME) and aqueous root extract (PAE) of Pluchea lanceolata exhibited 66.48% and 60.86% DPPH scavenging activity. The antioxidant activity of these extracts towards hydrogen peroxide was also reported. A 88.43% and 79.44% of inhibition of hydrogen peroxide was observed with PME and PAE respectively, when compared with control, at a concentration of 0.1 mg/ml using ascorbic acid as standard and positive control on analysis with UV-Visible Spectrophotometer. The results conclude that the extracts are a potential source of antioxidants of natural origin and may be a candidate for treating pathologies related to free radical oxidation due to its overall antioxidant effect in scavenging free radicals and active oxygen species.
Keywords:- Antioxidant, DPPH, free radical, Pluchea lanceolata, Asteraceae