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Research article:-Chemistry
* Wanare R. K.
Department of Chemistry, Jawaharlal Nehru College, Wadi, R.T.M., Nagpur University, Nagpur-23 (MS), India.
Abstract:- Compound 2,4-diacetyl phenol 1 has been prepared from 4-acetyl phenyl acetate by Fries rearrangement reaction and product 4-acetyl phenyl acetate was obtained from starting compound p-hydroxy acetophenone by using appropriate solvents as per reported literature. Product 2-(O-acetyl oximinoacetyl)-4-acetyl phenol 3 has been obtained via its acetylation of 2-oximinoacetyl-4-acetyl phenol 2 followed by oximation of 2,4-diacetyl phenol 1. Different 3-methyl-5-(3`-aryl prop-2`-enoyl)-1,2-benzisoxazoles 5a-o have been synthesized by the interaction of appropriate 3-methyl-5-acetyl-1,2-benzisoxazole 4 with different aromatic aldehydes and compound 4 was synthesized by the cyclisation of 2-(O-acetyl oximinoacetyl)-4-acetyl phenol 3 by using fresh dry pyridine. Oxidation of 3-methyl-5-(3`-aryl prop-2`-enoyl)-1,2-benzisoxazoles 5a-o with alkaline KMnO4 solution afforded 5-(3`-aryl prop-2`-enoyl)-1,2-benzisoxazole-3-carboxylic acids 6a-o. Glucuronidation of 6a-o with free D-gluconic acid by using dry pyridine to afford -D-glucuronosyl-5-(3`-aryl prop-2`-enoyl)-1,2-benzisoxazole-3-carboxylates 7a-o. Newly synthesized compounds are characterized by FT-IR, 1H NMR, FAB-MS, elemental analysis, TLC and their chemical properties.
Key Words:- 2, 4-Diacetyl phenol, 1,2-Benzisoxazole, Carboxylic acids and β-D-Glucuronides.
Original research article:-Microbiology
Sumathi K1*, Prakash M2, Lakshmi K3, Menezes GA3.
1Assistant Professor of Biochemistry, 2Assistant Professor of ENT, 3Assistant Professors of Microbiology, Sree Balaji Medical College and Hospital (Bharath University), Chennai, India.
Abstract:- Background: The risk of diabetic cochleopathy is proportionately increased with elevated glycated hemoglobin value. The aim of the study was to study the significance of HbA1c in deafness in type-2 diabetes mellitus. Material & Methods: Hundred diabetic patients attending a tertiary care hospital were included in the study. Serum HbA1c was estimated by ion-exchange chromatography & hearing loss by pure tone audiogram in all the patients. Results: Overall, 46 diabetic patients had sensori-neural hearing loss & 54 diabetic patients had normal hearing. This study found that the proportion of hearing loss among all diabetics was 46%, but in case of diabetic with poor glycemic control, it was 95.8%. Conclusion: Diabetic patients whose glycemic levels were not under control were more prone for hearing loss. Hence, Diabetic patients are advised to keep their glycemic levels under good control to prevent hearing loss. Screening for hearing loss can be recommended for the poorly controlled diabetic patients attending the outpatient department (OPD).
Key words:- HbA1c, Diabetes mellitus, Diabetic cochleopathy.
Original research article:-Biochemistry
*Renu Nagar
Department of Biochemistry,Dr. RP Govt. Medical College, Kangra, Himachal Pradesh, India.
Abstract:- Corpus luteal hormones and placental hormones stimulate glandular cells in target tissues bearing steroid hormone receptors to produce PSA. This PSA is detectable in female serum and shows variations reflecting the changes in levels of stimulating hormones. Predictably, therefore, the study found highest serum PSA during pregnancy, lesser during immediate postpartum period and least in non pregnant women. Serum PSA levels were found to show two peaks during menstrual cycle: a taller peak between 4th and 8th day of cycle and a smaller peak between 16th and 20th days. During pregnancy, higher serum PSA correlated to higher birth weight and male fetus. Postpartum serum PSA was higher in case of higher birth weight, higher gestation at delivery, male baby and instrumental delivery.
Key Words:- Prostate specific antigen, PSA, Menstrual cycle, Pregnancy, Post partum, Gestation, Gender, Birth weight, Type of delivery.
Research article:-Biology
Ahoyo Théodora A. (PhD) 1, Baba-Moussa Farid(PhD) 2, Adeoti Mansour F. (PhD) 3, Attolou Aimé G.(PhD) 4, Boco Martine (Msc) 4, Kotchoni Simeon O. (PhD) 5 and Baba-Moussa Lamine (PhD)6*
1Laboratoire de Biologie Humaine, EPAC/ Université d’Abomey-Calavi, 01 BP 526 Cotonou, BENIN.
2Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, ISBA-Champ de foire Cotonou, BENIN.
3Biochimie, RIPAQS International, Abidjan, CÔTE D’IVOIRE. 4Microbiology and Quality Assurance, CHDZ/ C Hospital, Abomey, BENIN.
5Department of biology and Center for Computational and Integrative Biology, Rutgers University, 315 Penn St., Camden, NJ 08102, USA.
6Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/ Université d’Abomey-Calavi, 05 BP 1604 Cotonou, BENIN.
Abstract:- Background: Increasing reports in the literature document the existence of nosocomial transmission of Serratia marcescens. The consequences of infections associated to this bacterium can be severe, so it was important to establish strategies for prevention. This study aimed to provide the prevalence of this bacterium in a hospital environment and to examine the main factors increasing the risk of cross contamination.
Methods: Various specimen obtained from 790 hospitalized children aged from 0 to 7 years were examined and 940 others samples taken from hands of medical personnel, and various hospital surfaces were also screened for Serratia marcescens presence. Susceptibilities to antimicrobial agents were tested by the disk diffusion method according to NCCLS guidelines. Aggressive infection control measures were instituted.
Results: About 123 (38%) patients were infected by Serratia marcescens, including septicaemia 65 (52.85%), 31 (25.2%) urinary tract infections, 12 (9.75%) pneumonia and 15 (12.2%) others infections. From hospital environment, 108/940 (11.50%) isolates were obtained. Antimicrobial susceptibility testing revealed that 56% of strains displaying multi resistance. Infected patients were cohorted and placed on contact precautions. Investigation by the infection control team revealed that the distributors of antiseptic were the main path of Serratia marcescens dissemination.
Conclusions: A substantial proportion of cases appeared to be of medical devices origin. New infection control policies and engineering plans were initiated on the basis of our results. Antimicrobial resistance is particularly harmful to infectious disease management in low-income countries since expensive second-line drugs are not readily available.
Key words:-Benin, Nosocomial infection, prevention, pediatrics unit, Serratia marcescens.
Original article:-Pharmaceutical sciences
Jain Nilesh *, Sharma Bhupendra Kumar, Jain Ruchi, Jain Deepak Kumar & Jain Surendra. *Sagar Institute of Research & Technology-Pharmacy,Ayodhya Bypass Road, Bhopal, M.P-462041, India.
Abstract:- A simple, precise, rapid and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) method is developed for the simultaneous estimation of Metoprolol Succinate (METO) and Telmisartan (TELM) present in multicomponent dosage forms. Chromatography is carried out isocratically at 25°C ± 0.5°C on an Prontosil C18 Column (5 µm,250mm x 4.60mm) with a mobile phase composed of acetonitrile: methanol: phosphate buffer pH-5 (35:35:30 % v/v/v) at a flow rate of 1.0 mL/min. Detection is carried out using a UV-PDA detector at 225 nm. Parameters such as linearity, precision, accuracy, recovery, specificity and ruggedness are studied as reported in the International Conference on Harmonization guidelines. The retention times for METO and TELM are 2.57 ± 0.5 min.and 4.68 ± 0.5 min. respectively. The linearity range for METO and TELM are 5-25g/ml & 8-40µg/ml and the recovery of added standards (80%, 100% and 120%) was in ranging from 98.24 to 99.35% for METO and 98.21 to 99.74% for TELM. The correlation coefficients for all components are close to 1. The relative standard deviations for three replicate measurements in three concentrations of samples in tablets are always less than 2%. The result obtained shows the developed method to be new (no method available for combination these drugs), rapid (Short retention time), simple, accurate (the value of SD and % RSD less then 2), precise and can be successfully employed in the routine analysis of these drugs in bulk drug as well as in tablet dosage form.
Key words:- RP-HPLC, Metoprolol succinate, Telmisartan, Simultaneous estimation.