DocumentsDate added
Original article
Preetinder Singh1,*,Yash Paul Dev Sharma2,Priyanka Singla3,Nitin Khuller1,Aman Bhatia1
Affiliation:-
1Associate Proffesor,2 Professor & Head,3PG Student, (Periodontology and Oral Implantology) SDD Hospital and Dental College, Barwala (Haryana), India
The name of the department(s) and institution(s) to which the work should be attributed:
Periodontology and Oral Implantology, SDD Hospital and Dental College, Barwala (Haryana) India
*Corresponding author:-
Dr. Preetinder Singh,MDS
Associate. Professor (Periodontology and Oral Implantology) SDD Hospital and Dental College, Barwala (Haryana), India
Contact number: +91-9915652946.
Abstract:
The increased rate and severity of periodontal destruction appear to be the result of the release and activation of increased quantities of inflammatory and tissue destructive molecules such as prostaglandins, cytokines, and matrix metalloproteinases (MMPs). Subantimicrobial dose doxycycline (SDD) has been shown to be an effective inhibitor of MMPs, especially collagenase. Aim and objective: To evaluate the adjunctive benefits of SDD along with surgical management of patients with the generalized aggressive periodontitis. Methods and material: Twenty subjects with aggressive generalized periodontitis received subgingival debridement along with conventional flap surgery and plus 6 months of adjunctive subantimicrobial doxycycline (SDD). Periodontal status was monitored at baseline, and at 1, 3 and 6 months after periodontal surgery. Results: The mean of baseline gingival index and on recall 3 came out to be clinically significant (p<0.05). The mean value of probing depth at baseline and that on recall 3 came out to be statistically significant (p<0.05) thus suggesting that there was considerable reduction in probing depth after conventional flap surgery along with SDD. The mean value of CAL at baseline and at recall 3 was clinically significant (p<0.05) thus suggesting that there was significant gain in CAL after conventional flap surgery along with SDD.
Conclusion: SDD can be used as a beneficial adjunct in the treatment of patients with aggressive periodontitis.
Keywords: Subantimicrobial dose doxycycline; Aggressive Periodontitis; Host Modulation.
REFERENCES
1.Newman, Takei, Klokkevold, Carranza; Clinical Periodontology; 10th Edition: Elseveir.
2.Page RC, Kornman KS. The pathogenesis of human periodontitis: An introduction. Periodontol 2000 1997; 14: 9- 11.
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5.Quirynen M, Mongardini C, De Soete M, et al. The role of chlorhexidine in the one-stage full-mouth disinfection treatment of patients with advanced adult periodontitis. Long-term clinical and microbiological observations. J Clin Periodontol 2000; 27: 578-589.
6.Caton JG, Ciancio SG, Blieden TM, et al. Treatment with subantimicrobial dose doxycycline improves the efficacy of scaling and root planing in patients with adult periodontitis. J Periodontol 2000; 71: 521-532.
7.Emingil G, Atilla G, Sorsa T, Luoto H, Kirilmaz L, Baylas H. the effect of adjunctive low dose doxycycline therapy on clinical parameters and gingival crevicular fluid matrix metalloproteinase 8 levels in chronic periodontitis. J Periodontol 2004; 75: 106-115.
8.Preshaw PM, Hefti AF, Novak MJ, Michalowicz BS, Pihlstrom BL, Schoor R et al. subantimicrobial dose doxycycline enhances the efficacy of scaling and root planing in chronic periodontitis: A multicentre Trial. J Periodontol 2004; 75: 1068- 1076.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: None
Article citation:
Preetinder Singh,Yash Paul Dev Sharma,Priyanka Singla,Nitin Khuller,Aman Bhatia. Efficacy of subantimicrobial dose Doxycycline as an adjunct to surgical therapy in patients suffering from aggressive periodontitis. J Pharm Biomed Sci 2014; 04(01): 29-33. Available at www.jpbms.info.
Copyright © 2014 Preetinder Singh,Yash Paul Dev Sharma,Priyanka Singla,Nitin Khuller,Aman Bhatia. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Bansal Sangeeta A1,*, Bansal Vinita A2
Affiliation:-
1Associated Professor, Department of Anesthesiology, 2Associate Professor, Department of Obstetrics and Gynecology, Index Medical College and Research Centre Indore, India.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Anesthesiology,
Index Medical College and Research Centre Indore, Madhya Pradesh, India
*Corresponding author:-
Dr. Sangeeta Bansal-Agarwal
401, Gold Residency, 7/2 Manoramaganj, Indore- 452001, India
Contact: +91-98260-15819
Abstract:
Background: Hysterectomies are associated with visceral and somatic pain. To get good perioperative pain relief various adjuvant are tried. Aim of our study was to find effects of low dose of intrathecal Clonidine as adjuvant to Bupivacaine in hysterectomies.
Material and methods: In a prospective randomized, double blind controlled trial, sixty patients were divided equally, randomly between two groups. Group I received 3.5 ml Bupivacaine 0.5% with 0.3 ml of normal saline. Group II received 3.5 ml Bupivacaine 0.5% with 0.3ml, 45 µg of Clonidine. Onset, duration of sensory and motor block, number of Diclofenac injection in first operative day along with haemodynamic, sedation, nausea, vomiting, shivering and other side effects were measured using f-test and t-test in data analysis package of the Microsoft Excel.
Results: Onset of sensory and motor block were comparable in two groups. There was significant increase in the duration of sensory block in Group II (in Group I was 186 minutes as compared to 206.8 minutes in Group II) (P value < 0.01). Similarly significant increase in duration of motor block in Group II (Group I was 208 minutes as compared to 240 minutes in Group II) (P value <0.03). Significant sedation was found in Group II (P value < 0.0001). Besides this all other parameters were comparable in two groups.
Conclusions: Low dose intrathecal Clonidine (45 µg) added to hyperbaric Bupivacaine increases duration and quality of block in hysterectomies with good sedation level and no side effects.
Keywords: Bupivacaine; Clonidine, Hysterectomy; Subarachnoid block.
REFERENCES
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2.Pedersen H, Santos AC, Steinberg ES, Schapiro HM, Harmon TW, Finster M. Incidence of visceral pain during cesarean section: The effect of varying doses of spinal bupivacaine. Anesth Analg 1990;69:46-9
3.David LB, Sinal,epidural and caudal anesthesia.In: Miller RD, editor.Miller Anesthesia. 6th ed ,Vol 2. Philadelphia : Churchill Livingstone;2005.p.1653-83.
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5.Cheney MA. Side effects of intrathecal and epidural opioids. Can J Anaesth 1995; 42:891-903.
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9.Quintin L, Viale JP, Annat G: oxygen uptake after major abdominal surgery: Effect of Clonidine. Anesthesiolgy 74 ; 236,1991.
10.Filos KS, Goudas LC, Patroni O, Polyzou V. Hemodynamic and analgesic profile after intrathecal Clonidine in humans: A dose response study. Anesthesiology 1994; 81:591-601.
11.Racle JP, Benkhadra A, Poy JY, Gleizal B. Prolongation of isobaric bupivacaine spinal anesthesia with epinephrine and Clonidine for hip surgery in the elderly. Anesth Analg 1987;66: 442-6.
12.Kaabachi O, Zarghouni A, Ouezini R, Abdelaziz AB, Chattaoui O, Kokki H. Clonidine 1 µg/kg is a safe and effective adjuvant to plain bupivacaine in spinal anesthesia in adolescents. Anesth Analg 2007; 105:516-9.
13.Gautier PE, De Kock M, Fanard L, Van Steenberge A, Hody JL. Intrathecal Clonidine combined with sufentanil for labor analgesia. Anesthesiology 1998;88:651-6.
14.Juliao MC, Lauretti GR. Low-dose intrathecal Clonidine combined with sufentanil as analgesic drugs in abdominal gynecological surgery. J Clin Anesth. 2000;12:357–62.
15.Dobrydnjov I, Axelsson K, Thorn SE, et al. Clonidine combined with small-dose bupivacaine during spinal anesthesia for inguinal herniorrhaphy: a randomized double-blinded study. Anesth Analg. 2003;96:1496–503.
16.Niemi L, Effects of intrathecal Clonidine on duration of bupivacaine spinal anaesthesia, hemodynamic and postoperative analgesia in patients undergoing knee arthroscopy. Acta Anesthesiol Scand 1994;38:724-8.
17.Acalovschi I, Bodolea C, Manoiu C. Spinal anaesthesia with meperidine. Effects of added α adrenergic agonists: Epinephrine vs Clonidine. Anesth Analg 1997;84:1333-9.
18.Fogarty DJ, Carabine UA, Milligan KR. Comparison of the analgesic effects of intrathecal Clonidine and intrathecal morphine after spinal anaesthesia in patients undergoing total hip replacement. Br J Anaesth 1993;71:661-4.
19.Grace D, Bunting H, Milligan KR, et al. Postoperative analgesia after co-administration of Clonidine and morphine by the intrathecal route in patients undergoing hip replacement. Anesth Analg. 1995; 80:86–91.
20.Dutta Dedjyoti, Naskar Chhandasi, Wahal Rita, Bhatia V K, Singh Vinita.intrathecal Clonidine for perioperative pain relief in abdominal hysterectomy. Indian Journal of Pain 2013; 27, 26-32.
21.Jeon YT, Jeon YS, Kim YC, Bahk JH, Do SH, Lim YJ. Intrathecal Clonidine does not reduce post-spinal shivering. Acta Anaesthesiol Scand 2005; 49:1509-13.
22.Sethi BS, Samuel M, Sreevastava D. Efficacy of analgesic effects of low dose intrathecal Clonidine as adjuvant to bupivacaine. Indian J Anaesth 2007; 51:415-9.
23.Thakur A, Bhardwaj M, Kaur Kiranpreet, et al.intrathecal Clonidine as an adjuvant to hyperbaric Bupivacaine in patients undergoing inguinal herniorrhaphy: Journal of Anesthesiolgy Clinical pharmacology 2013;29;66-70.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Department of Anesthesiology, Index Medical College Indore (M P).
Article citation:-
Bansal Sangeeta A,Bansal Vinita A. Efficacy of intrathecal Clonidine in perioperative pain relief in Hysterectomies. J Pharm Biomed Sci 2014; 04(01): 09-13. Available at www.jpbms.info.
Copyright © 2014 Bansal Sangeeta A, Bansal Vinita A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Brief Communication
Harpreet Kaur1,*, Shalini Gupta2
Affiliation:-
1Assistant Professor,2Associate Professor, Department. of Biochemistry, Gian Sagar Medical College and Hospital, Ram Nagar, Rajpura, Distt. Patiala, Punjab, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Biochemistry, Gian Sagar Medical College and Hospital, Ram Nagar, Rajpura, Distt. Patiala, Punjab, India
*Corresponding author:-
Dr. Harpreet Kaur.
Assistant Professor, Department of Biochemistry, Gian Sagar Medical College and Hospital, Ram Nagar, Rajpura, Distt. Patiala, Punjab, India
Contact number: +91-9915022170
Abstract:
Thyroid function tests are designed to distinguish hyperthyroidism and hypothyroidism from the euthyroid state. Thyroid stimulating hormone (TSH) in the body follows a circadian rhythm, i.e., it follows a 24-hour cycle with regular fluctuations. TSH is nadir in the late afternoon and peak at midnight. As subclinical hypothyroidism diagnosis can be missed if serum sample for thyroid hormones is estimated in an afternoon and it is a risk factor for various diseases, so an early morning fasting sample should be preferred choice of estimation.
Keywords: Thyroid hormones; Fasting; Non fasting; Diagnosis; Subclinical hypothyroidism.
Harpreet Kaur, Shalini Gupta. Diagnostic significance of serum thyroid hormones level estimation in early morning fasting sample. J Pharm Biomed Sci. 2014; 04(01):55-57. Available at www.jpbms.info.
REFERENCES
1.Brabant G, Prank K, Ranft U, Schuermeyer Th, Wagner TOF, Hauser H, et al. Physiological regulation of circadian and pulsatile TSH secretion in normal man and woman. J Clin Endocrinol Metab. 1990; 70(2):403–7.
2.Weeke J and Gundersen HJ. Circadian and 30 minutes variations in serum TSH and thyroid hormones in normal subjects. Acta Endocrinol 1978; 89: 659-672.
3.Scobbo RR, VonDohlen TW, Hassan M and Islam S. Serum TSH variability in normal individuals: the influence of time of sample collection The West Virginia Medical Journal 2004; 100(4):138-142.
4.Sviridonova MA, Fadeyev VV, Sych YP and Melnichenko GA. Clinical significance of TSH circadian variability in patients with hypothyroidism. Endocr Res. 2013; 38 (1), 24-31.
5.Bandyopadhyay D, Goel P, Baruah H and Sharma D. Fasting or random: which venous blood sample is better for thyroid testing? J. Adv Researches in Biol Scienc.2012; 4(4): 275-278.
6.Biondi B and Cooper DS. The clinical significance of subclinical thyroid dysfunction. Endocr Rev 2008; 29: 76-131.
7.Ochs N, Auer R, Bauer DC, Nanchen D, Gussekloo J, Cornuz J et al., Meta-analysis: subclinical thyroid dysfunction and the risk for coronary heart disease and mortality. Ann Intern Med 2008; 148: 832-845.
8.Tian L, Song Y, Xing M, Zhang W, Ning G, Li X et al., A novel role for thyroid-stimulating hormone: up-regulation of hepatic 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase expression through the cyclic adenosine monophosphate/protein kinase A/cyclic adenosine monophosphate-responsive element binding protein pathway. Hepatology 2010; 52: 1401-1409.
9.Xing Wanjia, Wang Chenggang, Wang Aihong, Yang Xiaomei, Zhao Jiajun, Yu Chunxiao et al., Lipids in Health and Disease 2012; 11: 44-47.
10.Ruhla S, Weickert MO, Arafat AM, et al. A high normal TSH is associated with the metabolic syndrome. Clin Endocrinol (Oxf ) 2010; 72: 696-701.
11.Oh JY, Sung YA and Lee HJ. Elevated thyroid stimulating hormone levels are associated with metabolic syndrome in euthyroid young women. Korean J Intern Med 2013; 28: 180-186.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: None.
Copyright © 2014 Harpreet Kaur, Shalini Gupta. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
Tabbouche O.S.*, Soukkariyyeh I., & Alwan T.
Affiliation:-
New Mazloum Hospital, Tripoli, Lebanon. Department of Pharmaceutical Quality Control, Research Laboratory, Faculty of Pharmacy, Jinan University Of Lebanon, Tripoli, Lebanon.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Pharmaceutical Quality Control, Research Laboratory, Faculty of Pharmacy, Jinan University Of Lebanon, Tripoli, Lebanon.
*Corresponding author:-
Omar S Tabbouche
Department of Pharmaceutical Quality Control, Research Laboratory, Faculty of Pharmacy, Jinan University Of Lebanon, Tripoli, Lebanon.
Abstract:
Meropenem is a Carbapenem antibiotic having a broad spectrum of activity against the majority of Gram negative, Gram positive, and anaerobic bacteria. Recently, carbapenemase-producing bacteria (Klebsiella pneumonia and Acinetobacter bomanii) have emerged showing resistance to all carbapenems including Meropenem. The treatment of critical infections caused by such microorganisms has become challenging to Infectious Diseases Specialists throughout the globe. The Meropenem continuous IV infusion protocol showed better pharmacodynamics profile which increases effectiveness of Meropenem for the treatment of such infections. In the present study, we have developed a UV-spectrophotometric protocol for the determination of Meropenem stability after reconstitution with Normal Saline at room temperature. We have also compared the stability of different Meropenem brands present in the Lebanese market together with the percentage of the Active Ingredient as compared to the Originator product Meronem®(Astrazeneca™). Our samples included Meropenem formulations from 5 different brands: Meronem® (Comparator formulation), Meropenem Hospira ®, Meropenem Labatec ®, Ropenem®, & Aropem®. All products showed similar aspects of raw material purity except Ropenem® which has showed a different curve pattern. In the quantitative testing, the highest absorbance reading corresponded to Meropenem Labatec®, while the lowest corresponded to Ropenem®. The product having the nearest concentration to the originator product is Aropem®. In stability testing, during the 3 hours interval, only Meronem® & Aropem® have been stable, where as the rest showed more than 10% degradation. During the 8 hours interval, all Meropenem brands have showed much greater than 10% degradation.
Keywords: Meropenem; Degradation.
REFERENCES
1.Cielecka-Piontek J., Paczkowska M., Lewnadowska K., Barszcz B., Zalewski P., and Garbacki P.Solid-state stability study of Meropenem – solutions based on spectrophotometric analysis. Chem Cent J 20137(1): 98.
2.United States Pharmacopeia and National Formulary (USP 29 NF 24). Supplement No. 2. Rockville, MD: United States Pharmacopeia Convention; 2006: 3711.
3.Shah V.A., Rathod S.M., Parmar R.R., & Shah D.A. Development and validation of analytical method for Meropenem in pharmaceutical dosage form. Inter Nat Jour Inst Phar Life Sci 2012; 503-509.
4.Taccone F.S. Continuous infusion of meropenem in critically ill patients: practical considerations. Critical Care 2012; 16, 444.
5.Berthoin K., Le duff C.S., Marchand-Brynaert J., Carryn S., and Tulkens P.M. Stability of Meropenem and doripenem solutions for administration by continuous infusion. J Antimicrob Chemother 2010;1073-1075. doi 10.1093/jac/dkq044.
6.Jaruratanasirikul S. and Sriwiriyajan S. Stability of Meropenem in normal saline solution after storage at room temperature. Southeast Asian J Trop Med Public Health 2003; 34(3): 627-629.
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8.European Pharmacopoeia (6th edition). Volume II. European Pharmacopoeia Commission, Strasbourg, France, 2008.
9.British Pharmacopoeia CD version 2. The British Pharmacopoeia Commission. London, 2009.
10.Stability Statements of Meropenem in ANAPA Ambulatory Infusion Device. Supramol™ Parenteral Colloids GmbH. www.anapa.com/board/config/download.php?fid=21.
Article citation:-
Tabbouche O.S., Soukkariyyeh I., & Alwan T. Development of a UV-spectrophotometric protocol for the determination of Meropenem stability over 8 hours in solutions for IV infusion at room temperature and quantitative/stability comparison of marketed brands. J Pharm Biomed Sci 2014;04(01):04-08. Available at www.jpbms.info.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Copyright © 2014 Tabbouche O.S., Soukkariyyeh I., & Alwan T. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case report
S. H. Arif1, S. S. Ahmad1, Kafil Akhtar2,*, R. S. Chana3
Affiliation:-
1Professor,2Associate Professor,Department of Pathology, 3Professor,Department of Surgery, J. N. Medical College, A. M. U. Aligarh (UP):202002,India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Pathology,
J. N. Medical College, A. M. U. Aligarh (UP): 202002, India
Author contributions: All the authors contributed equally to this paper.
*Corresponding author:
Dr. Kafil Akhtar.
Associate Professor, Department of Pathology,
J. N. Medical College, A. M. U. Aligarh (UP): 202002, India
Abstract:
Cystic partial differentiated nephroblastoma (CPDN) is rare multilocular cystic lesion of kidney having low malignant potential. It is differentiated from multilocular cystic disease of kidney by the presence of partially or well differentiated renal element or renal balstemal cell in the septa of the locules of the cyst. It is treated with partial nephrectomy with 100% survival rate but chance of recurrence is high and therefore regular follow up is necessary. CPDN occurs in children of less than 2 years of age but adult cannot be spared. We report an unusual case of CPDN in a nine year old female who presented with abdominal lump for a period of 2 months.
Keywords: Cystic partial differentiated nephroblastoma; Cystic lesion of kidney; Female.
Article citation:
S. H. Arif, S. S. Ahmad Kafil Akhtar, R. S. Chana. Cystic partially differentiated nephroblastoma – a rare entity – a case report. J Pharm Biomed Sci 2014; 04(01): 58-60. Available at www.jpbms.info.
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9.Tiryaki T, Hucumenoglu S, Livanelioglu Z, Atayurt H. Cystic partially differentiated nephroblastoma – a case report. Urol Int 2003; 70: 223-226.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: None
Copyright © 2014 S. H. Arif,S. S. Ahmad,Kafil Akhtar, R. S. Chana. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.