DocumentsDate added
Original article
Hitesh R Ahir*,Niraj Kumar Biswas, Parimal H Patel, Mehul R Patel
Affiliation:-
Department of Microbiology, GMERS Medical College & Hospital, Valsad, Gujarat, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, GMERS Medical College & Hospital, Valsad, Gujarat, India
Address reprint requests to
Hitesh R Ahir,
Department of Microbiology GMERS Medical College & Hospital, Valsad, Gujarat, India
Article citation:
Ahir HR, Bisvas NK, Patel PH, Patel MR. Prevalence of Pseudomonas infection and its antibiotic susceptibility pattern isolated from various clinical samples at tertiary care hospital, Valsad, Gujarat. J Pharm Biomed Sci. 2014;04(12):1039-1041. Available at www.jpbms.info
ABSTRACT
Objectives: Pseudomonas is one of the important bacterial pathogens isolated from various clinical samples. Several studies indicate that resistance patterns are increasing nowadays. The present study was done to assess the Prevalence of Pseudomonas infection and antibiotic susceptibility pattern of pseudomonas isolated from various clinical samples.
Materials and Methods: A total of 697 samples were cultured out of them 71 pseudomonas isolates from different clinical samples, e.g. urine, pus.csf, sputum, and blood were tested for antibiotic susceptibility pattern using modified disk diffusion method as per Clinical and Laboratory Standards Institute guideline (CLSI)
Results: Prevalence of Pseudomonas infection is 10.18% detected at GMERS medical college and Hospital, Valsad, Gujarat, India. Major part of Pseudomonas isolates were detected from wounds.
Conclusion: Prevalence of Pseudomonas is higher in surgical patients and Multi-drug resistant isolates are also present in this institute.
KEYWORDS: Pseudomonas, Kirbey bour disk diffusion method, Antibiotic susceptibility pattern.
REFERENCES
1.Ramana B.V, Chaudhury A. Antibiotic resistance pattern of Pseudomonas aureuginosa isolated from healthcare associated infections at a tertiary care hospital. J Scientic Society 2012; 39 (2):78-80.
2.Shenoy S, Baliga S, Saldhana D, Prashanth HV. Antibiotic Sensitivity Patterns of Pseudomonas aeruginosa Strains isolated from various clinical specimens. Ind J of Med Sciences 2002; 56 (9):427-30.
3.Arora D, Neerja J, Rajiv K, Romit. Emerging Antibiotic Resistance in Pseudomonas-A Challenge. Int J Pharm Pharm Sci. 2011; 3(2): 82-4.
4.Pathmanathan SG, Samat NA, Mohamed R. Antimicrobial susceptibility of clinical isolates of Pseudomonas aeruginosa from a Malaysian Hospital. Malay J Med Sci 2009; 16(2):28-33.
5.Lambert P A. Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J R Soc Med 2002; 95(suppl 41): 22-26.
6.Babay H A H. Antimicrobial Resistance among Clinical Isolates of Pseudomonas aeruginosa from patients in a Teaching Hospital, Riyadh, Saudi Arabia, 2001-2005. Jpn J Infect. Dis 2007;
60:123-125.
7.Ergin C, Mutlu G. Clinical distribution and antibiotic resistance of Pseudomonas species.Eastern Journal of Medicine 1999; 4(2): 65-69.
8.Holloway WJ, Palmer D. Cinical application of new parenteral antibiotic in treatment of severe bacterial infection. Am J Med 1996; 525-595.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014 Ahir HR, Biswas NK, Patel PH, Patel MR. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Devinder Singh Negi1, Pankaj Shrivastava2, Smruti Prava Das1,*
Affiliation:-
1Department of Chemistry, Ravenshaw University, Cuttack - 753003, Odisha, India
2DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar - 470001, Madhya Pradesh, India
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Chemistry, Ravenshaw University, Cuttack - 753003, Odisha, India
2. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar - 470001, Madhya Pradesh, India
Address reprint requests to
Dr.Smruti Prava Das.
Department of Chemistry, Ravenshaw University, Cuttack - 753003, Odisha, India
Article citation:
Negi DS, Shrivastava P, Das SP. Microsatellite markers alleles analysis in 12 cases of paternity establishment by DNA Profiling. J Pharm Biomed Sci. 2014; 04(12):1057-1071. Available at www.jpbms.info
ABSTRACT
The paternity is established by examination of inherited alleles in the questioned child from the reference parent samples. The alleles for all examined microsatellite loci unambiguously assigned to the questioned child as maternal and paternal alleles. In this study, we have examined 11 family trios and 1 family with 4 trios and convincingly established the paternity in 12 cases by DNA profiling. This study present an extensive analysis of number of possible alleles, homozygous and heterozygous alleles, inheritance pattern of maternal and paternal alleles in child, paternity index and probability of paternity in all the 12 cases. The total numbers of alleles in 15 microsatellite loci are 182. The average percent numbers of homozygous and heterozygous loci were 21.63 and 78.37 respectively. The likelihood of first allele in the child inherited from mother and fathers were 6.8 and 8.2 respectively. The average cumulative paternity index and probability of paternity were 1124182187 and 0.999999859 respectively in all the 12 cases. The analysis of STR alleles of microsatellite markers inferred the high heterozygosity, around equal chance that first inherited allele is from either parent, and high average values of cumulative paternity index and probability of paternity. The sample size of 12 cases wherein 15 trio are examined exhibited the robustness, efficiency and accuracy of markers with high diverse allele pool for establishment of paternity. The study showed the application of allele frequency database of Indian population, probability value and statistical methods in paternity establishment cases.
KEYWORDS: microsatellite, alleles; homozygosity; heterozygosity; paternity index; probability.
REFERENCES
1.Jeffreys AJ, Wilson V and Thein SL. Hypervariable ‘minisatelite’ regions in human DNA. Nature. 1985;314:67-73.
2.Wang DY, Chang CW, Lagac RE, Calandro LM, Hennessy LK. Developmental Validation of the AmpFlSTR® Identifiler® Plus PCR Amplification Kit: An Established Multiplex Assay with Improved Performance. J Forensic Sci 2012;2(57):453-65.
3.Maniatis T, Fritsch EF, Sambrook J, Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press. 1982.
4.Negi DS, Das SP. Quantitative and qualitative chemical extraction of Deoxyribo Nucleic Acid DNA from human cell organelles. Res J Chem Sci. 2014;4(8):75-81.
5.Negi DS, Shrivastava P, Das SP. DNA sequencing by polymer synthesis with variable ratio of deoxynucleotide triphosphate and fluorescent dideoxynucleotide triphosphate. Asian J of Biomed and Pharma Sci. 2014;4(32):32-8.
6.Negi DS, Das SP. Computational chemical analysis of DNA sequencing by reducing graphene oxide with the released H ion during polymer synthesis. J of Chem and Pharma Res 2014;6(7):2190-2196.
7.Primorac D, Schanfield MS, Primorac D. Application of forensic DNA testing in the legal system. Croat Med J. 2000;41(1):32-46
8.Negi DS, Alam M, Bhavani SA, Nagaraju J. Multi-step microsatellite mutation in maternally transmitted locus D13S317: A case of maternal allele mismatch in the child. Int J of Legal Med. 2006;120:286-92.
9.Shrivastava P, Neetu M, Sharma NC, Trivedi VB, Negi DS, Verma MK. Autosomal STR genotyping analysis of juvenile delinquents of Madhya Pradesh: A pilot Study. Adv Bio Tech. 2013;13:20-4.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014 Negi DS, Shrivastava P, Das SP. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case report
Sunil Kumar1,*, VaibhaoGabhane2, Sanjay K Diwan1
Affiliation:-
1Professor,2Resident, Department of Medicine, Jawaharlal Nehru Medical College, DMIMS (DU), Sawangi (Meghe), Wardha, Maharashtra, India.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Medicine, Jawaharlal Nehru Medical College, DMIMS (DU), Sawangi (Meghe), Wardha, Maharashtra, India
Address reprint requests to
Sunil Kumar.
Professor, Department of Medicine, Jawaharlal Nehru Medical College, DMIMS (DU), Sawangi (Meghe), Wardha, Maharashtra, India
Article citation: Kumar S, Vaibhao GS, Sanjay DK. Leptospirosis induced acute kidney injury in elderly: It’s different?. J Pharm Biomed Sci.2014; 04(12):1103-1105. Available at www.jpbms.info
ABSTRACT:
Leptospirosis is very important zoonosis in the world. Patients are typically young men. Here we report a 68-year-old healthy elderly man presented to our hospital with flu like syndrome and acute kidney injury. Eventually, Ig M Antibody for leptospira was found positive. Antibiotic treatment is efficient in the early and late or severe phases. In this case early and daily hemodialysis leads to rapid recovery of the patient.
KEYWORDS: Leptospirosis, acute kidney injury, elderly.
REFERENCES
1.Vijayachari P, Sugunan AP, Shriram AN. Leptospirosis: an emerging global public health problem. J Biosci. 2008;33:557–656.
2.Kobayashi Y. Human leptospirosis: management and prognosis. J Postgrad Med.2005;51:201–4.
3.Gancheva G I. Leptospirosis in elderly patients. braz j infect dis. 2013;17(5):592–595.
4.Sambasiva RR, Naveen G, Bhalla P, Agarwal SK. Leptospirosis in India and the rest of the world. Braz J Infect Dis. 2003;7:178-93.
5.Muthusethupathi M A, Shivakumar S, Vijayakumar R, Jayakumar M. Renal involvement in leptospirosis--our experience in Madras City. J Postgrad Med 1994;40:127
6.Abdulkader RCRM, Silva MV. The kidney in leptospirosis. PediatrNephrol 2008;23:2111-20.
7.Sitprija V, Losuwanrak K, Kanjanabuch T. Leptospiral nephropathy. SeminNephrol 2003;23:42-8.
8.L. Andrade, E. de Francesco Daher, A.C. Seguro. Seminars in Nephrology 2008;28(4):383-394.
Source of support: None.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Kumar S, Vaibhao GS, Sanjay DK. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case report
Navpreet Kaur Sandhu1, Amandeep Kaur Multani1,Saurabh Bither3, Priya Gupta2,Shruti Gupta2,*,Tejveer Singh3,¥
Affiliation:-
1BDS Intern,2Senior lecturer,Department of Oral and Maxillofacial Pathology, Luxmi Bai Institute of Dental Sciences and Hospital, Patiala, Punjab, India
3Reader,3¥Senior lecturer,Department of Oral and Maxillofacial Surgery, Luxmi Bai Institute of Dental Sciences and Hospital, Patiala, Punjab, India,
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Oral and Maxillofacial Pathology, Luxmi Bai Institute of Dental Sciences and Hospital, Patiala, Punjab, India
Address reprint requests to
Priya Gupta,
577/4A Ekta Vihar,
Anand Nagar B,Patiala, Punjab, India
ABSTRACT
Oral mucosa is constantly subjected to external and internal stimuli and therefore manifests a spectrum of disease that range from developmental, reactive, and inflammatory to neoplastic. These lesions present either as generalized or localized conditions. Localized hyperplastic lesion of the gingiva or ‘epulide’, a well-recognized clinical entity is used to designate all discrete tumors and tumor-like masses of gingiva. We present a case where patient had a gingival hyperplastic lesion on the lingual aspect of anterior mandibular teeth.
KEYWORDS: Epulide; Hyperplastic; Reactive.
REFERENCES
1.Willies-Jacobo LJ, Isaacs H Jr, stein MT. Pyogenic granuloma presenting as a congenital epulis. Arch Pediatr Adolesc Med. 2000;154(6):603-5.
2.Neville BW, Damm DD, Allen CM, Bouquot JE. Oral and Maxillofacial Pathology. 3rd ed. Missouri: Saunders; 2009.
3.Regezi JA, Sciubba JJ, Jordan RCK. Oral pathology: clinical pathologic correlations. 2012. 6th ed. St. Louis, Missouri, Saunders.
4.Panseriya BJ, Hungund S. Pyogenic granuloma associated with periodontal abscess and bone loss - A rare case report. Contemp Clin Dent. 2011; 2(3): 240–4.
5.Vilman A, Vilman P, Vilman H. Pyogenic granuloma: evaluation of oral conditions. Br J Oral Maxillofac Surg. 1986;24(5):376-82.
6.Kamran Ali, Muhammad Rafique Chatha, Navid Rashid, Mahwish Raja. Pyogenic granuloma- review. Pakistan Oral and Dent. 2006;26(1):59-62.
7.Jafarzadeh H, Sanatkhani M, Mohtasham N. Oral pyogenic granuloma: a review. J Oral Sci 2006;48(4):167–75.
8.Sharma P, Khan MH, Kumar N, Samadi FM. Pyogenic granuloma: A case report and review. Journal of Dentofacial sciences. 2013;2(3):9-11.
9.Parisi E, Glick PH, Glick M. Recurrent intraoral pyogenic granuloma with satellitosis treated with corticosteroids Oral Dis. 2006;12(1):70-2.
10.Shafer, Hine, Levy. Shafer’s textbook of oral pathology. 2009. 6th ed. India: Elsevier.
11.Ichimiya M, Yoshikawa Y, Hamamoto Y, Muto M. Successful treatment of pyogenic granuloma with injection of absolute ethanol. J Dermatol 2004;31(4):342-4.
12.Souza LN, Martins CR, de Paula AM. Cutaneous horn occurring on the lip of a child. Int J Paediatr Dent. 2003;13(5):365-7.
13.Kerr DA. Granuloma Pyogenicum. Oral Surg Oral Med Oral Pathol. 1951;4(2):158-76.
14.Wang SQ, Goldberg LH. Treatment of recurrent pyogenic granuloma with excision and frozen section for margin control. Dermatol Surg. 2008;34(8):1115-6.
15.Abdulai A.E, Nuamah I.K, Baddoo H, Gyasi R.K. Oral pyogenic granuloma in Ghanaians: a review of cases. Int J Med Biomed Res. 2013;2(3):173 -8.
16.Eversole Lr. Clinical Outline of Oral Pathology: Diagnosis And Treatment, 3rd Ed. 2002. Bc Decker, Hamilton.
17.Michael W. Finkelstein. A Guide to Clinical Differential Diagnosis of Oral Mucosal Lesions. 2013. Continuing Education Course.
18.Prasanna JS, Sehrawal S. Fibroepithelial hyperplasia: rare, selflimiting condition- two case reports. J Oral Research. 2011;2:63-70.
Article citation:
Sandhu NK, Multani AK, Bither S, Gupta P, Gupta S, Singh T. Gingival Growths: A diagnostic enigma. J Pharm Biomed Sci. 2014;04(12):1079-1083. Available at www.jpbms.info
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014 Sandhu NK, Multani AK, Bither S, Gupta P, Gupta S, Singh T. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Vijaya Mahanth Prasad.K 1,*,Vijay Mahantesh Sunkad 2,Jyothi Gopal3,Chethana. K.V4
Affiliation:-
1,2Senior Resident, Department of Orthopedics, 3Senior Resident, Department of Anesthesia, 4Post Graduate, Department of Community Medicine,Navodaya Medical College Hospital, Raichur, Karnataka, India
The name of the department(s) and institution(s) to which the work should be attributed:
Navodaya Medical College Hospital, Raichur, Karnataka, India
Address reprint requests to
Dr Vijay Mahantesh.
Senior Resident, Department of Orthopedics, Navodaya Medical College Hospital, Raichur, Karnataka, India
Article citation:
Prasad VM, Sunkad VM, Gopal J, Chetnana KV. Functional outcome of fracture neck femur treated with bipolar hemiarthroplasty. J Pharm Biomed Sci. 2014;04(12):1050-1056. Available at www.jpbms.info
ABSTRACT
Background: Hip fractures are common and comprise about 20% of the operative workload of an orthopedic trauma unit. Intracapsular femoral neck fractures account for about 50% of all hip fractures. The objective of treatment of femoral neck fractures in the mobile elderly population is the early restoration of premorbid walking ability and quality of life. Hemiarthroplasty (Unipolar or Bipolar) is the most common treatment for displaced fractures of the femoral neck in the elderly and is associated with better functional outcome and fewer reoperations than internal fixation.
Aims and Objectives: To study the results of bipolar prosthesis with respect to pain, mobility and stability.
Methodology: The present study was conducted in the department of Orthopaedics, on patients who had sustained an intracapsular femoral neck fractures during the period of October 2011 to October 2012.
Results: The average age of the patients was 70.4years. The size of the prosthesis used varied from 39 mm to 49 mm. The fracture was managed by Bipolar hemiarthroplasty, we have excellent results in 10 cases (33.33%), good in 11cases (36.67%), fair in 7 cases (22.33%) and poor in two cases (6.67%) according to the Harris hip rating system. The pain was analysed at 6 weeks, 3 months and 6 months follow up according to Visual analogue scale (VAS). At 6 months follow up period, 70 % patients had no pain (0), 20 % had mild pain (1-4), 6.67 % moderate pain (5-8) and 3.33 % severe pain (>8).
KEYWORDS: Hip fractures; Bipolar hemiarthroplasty; Visual analogue scale.
REFERENCES
1.Singer BR, McLauchlan GJ, Robinson CM, et al. Epidemiology of fractures in 15,000 adults: the influence of age and gender. J Bone Joint Surg (Br) 1998;80(2):243-248.
2.Dennison E, Mohamed MA, Cooper C. Epidemiology of osteoporosis. Rheum Dis Clin North Am 2006;32(4):617-629. 4.
3.Speed K. Fractures: 50 year review of teaching and treatment. Illinois Med J 1952; 102:85-92.
4.Lu-Yao GL, Keller RB, Littenberg B, Wennberg JE. Outcomes after fractures of the femoral neck: A meta analysis of 106 reports. J Bone Joint Surg 1994; 76(A): 15-25.
5.JE, Magaziner J, Hudson J, Hebel JR, Young Y, Hawkes W et al. Outcome after hemiarthoplasty for femoral neck fractures in the elderly. Clin Orthop. Relat Res. 1998; 348: 51-8.
6.Frihagen F, Nordsletten L, Madsen JE. Hemiarthroplasty or internal fixation for intracapsular displaced femoral neck fractures: randomised controlled trial. BMJ. 2007;335:1251–1254.
7.Parker MJ, Gurusamy K. Internal fixation versus arthroplasty for intracapsular proximal femoral fractures in adults. Cochrane Database Syst Rev. 2006;4:CD001708.
8.Rogmark C, Johnell O. Primary arthroplasty is better than internal fixation of displaced femoral neck fractures: a meta-analysis of 14 randomized studies with 2,289 patients. Acta Orthop 2006; 77: 359-367.
9.Rogmark C, Carlsson A, Johnell O, Sembo I. Costs of internal fixation and arthroplasty for displaced femoral neck fractures: a randomized study of 68 patients. Acta Orthop Scand 2003; 74: 293-298.
10.Keating JF, Grant A, Masson M, et al. Randomized comparison of reduction and fixation, bipolar hemiarthroplasty, and total hip arthroplasty. Treatment of displaced intracapsular hip fractures in healthy older patients. J Bone Joint Surg (Am). 2006; 88: 249-260.
11.Ries;bipolar hemiarthroplasty for recurrent dislocation after THA a report of 3 cases Ries M D ,Wiedel J D Clin Orthop Relat Res 1992 Ray(278):121-7
12.Giliberty RP: Bipolar endoprosthesis minimizes protrusio acetabuli, loose stems. J Bone Joint Surg 1985; 67B:3, 13.
13.Mannarino F,Maples D,Colwill JC &Swanson AB,Bateman Bipolar Hip Arthroplasty;A Review of 44 cases.Orthopaedics 1986 March;9(3):357-60.
Source of support: None.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Prasad VM, Sunkad VM, Gopal J, Chetnana KV. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.