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Original article
Sania A. Shaddad1,*, Salah El Din Abdel Hag2, Tigani Hassan3, Sumaya I Abass4
Affiliation:
11Department of Pharmacology, Faculty of Medicine, University of Khartoum, Sudan
2Department of Pharmacology, College of Medicine, University of Bahr Elghazal, Sudan
3Department of Medicine, Pharmacology & Toxicology, Faculty of Veterinary Medicine University of Khartoum, Sudan
4Microbiology- Veterinary Research Centre, Khartoum Sudan
Address reprint requests to
Prof Sania A Shaddad.
Department of Pharmacology Faculty of Medicine, University of Khartoum, Al-Gamaa Ave, Khartoum 11111, Sudan
Article citation: Mohammed Hamad AE, Sania Shaddad AI, Mohammed OY. Investigation of in vitro super growth generation of E. coli by ox bile. J Pharm Biomed Sci. 2015 Feb; 05(02) Suppl:S39-S42. Available at www.jpbms.info
ABSTRACT:
Ox bile as such has ethno-pharmacological use including infectious conditions. This therapeutic use was verified experimentally. Fresh ox bile was collected under aseptic conditions. Pathogenic isolates of Escherichia coli were cultivated using standard microbiological methods. Antimicrobial actions of ox bile were tested on standard pathogenic Escherichia coli cultured on blood agar. Whole ox bile & 50 % were bacteristatic to the microorganism. At the concentration of 33%, ox bile generated supergrowth of the microorganism. The microorganism was not sensitive to 25% concentration of ox bile.
KEYWORDS: Escherichia coli; super-growth; ox bile.
REFERENCES
1.Abdel Hag, S. E. A. (2008). A pharmacoligical study of some therapeutic & antimicrobial potentials of ox bile, PhD. Thesis, University of Khartoum, Sudan.
2.Bockus, H. L. (1953). Affections of the gallbladder and bile ducts, Gastro-entrologly W. B. Saunders, Philadelphia: vol III. P. 424 – 715.
3.Collee, J. G.; Fraser, A. G., Marmion, B. P. and Simmons, A. (1996). Practical Medica lMicrobiolgy. 14th ed. Churchill Livingstone Inc. 650 Avenue of the Americas, New York, N.Y. 10011, USA.
4.Elsanousi, S.M.; Ali, B.H. and El Sheikh, A. (2004). The influence of deoxycholic acid on the inhibitory effect of Furazolidone in vitro, (unpublished paper).
5.Garriga, Paseual. Selection of lactobacilli for chicken probiotic adjuncts, Journal of applied microbiology 1997; 84,1:125.
6.Hulitanen, C.M. Bile acid inhibition of Clastridium botulinum, Applied Environmental microbiology 1979; 38: 216 – 218.
7.Kalashinic, S.A. Bactericidal action of human and bovine bile on Staphylococci, Varachebonoe Delo.1973;10:132 -154.
8.Tkachuch, N.I. Protection of antimicrobial activity of Furazolidone by bile acids. Vrachebonoe Delo 1983; 4:110 – 112.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Shaddad AS et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Sanjay Kumar Porwal1,*,B.C.Mewara1,¥,Madhusudan Swarnkar1,€,Sanjeev Gupta1,ß
Affiliation:
1,*Associate Professor, 1,¥Assistant Professor, Department of Surgery, Jhalawar Hospital and Medical College Society, Jhalawar (Rajasthan)-326001, India
1,€ Assistanrt Professor, Department of P.S.M, Jhalawar Hospital and Medical College Society, Jhalawar (Rajasthan)-326001, India
1,ß Senior Medical Officer, Department of Anaesthesia, Jhalawar Hospital and Medical College Society, Jhalawar (Rajasthan)-326001, India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Surgery, Jhalawar Hospital and Medical College Society, Jhalawar (Rajasthan)-326001, India
2.Department of P.S.M, Jhalawar Hospital and Medical College Society, Jhalawar (Rajasthan)-326001, India
3.Department of Anaesthesia, Jhalawar Hospital and Medical College Society, Jhalawar (Rajasthan)-326001, India
Address reprint requests to
* Dr Sanjay Kumar Porwal.
B4 Anand Vihar, Jhalawar, Rajasthan- 326001, India
Article citation: Porwal, SK.,Mewara, BC.,Swarnkar, M,Gupta, S. A clinical study of enteric perforation peritonitis. J Pharm Biomed Sci. 2015 Feb;05 Suppl:S1-S4. Available at www.jpbms.info
ABSTRACT: Introduction: Enteric perforation is a a common occurrence in developing world. Its surgical complication is perforation peritonitis. It causes great morbidity and mortality and a socio economic burden to already poorer countries and developing countries.
Methodology: A retrospective study conducted at S.R.G. Hospital attached to Jhalawar Hospital and Medical College, Jhalawar (Rajasthan). It includes 100 cases. Data was collected and master chart prepared.
Results: This study shows that males are more affected. There is a lot of morbidity and mortality.
Conclusion: The commoner entity enteric perforation peritonitis can be put under control in terms of morbidity and mortality by better pre operative , per operative and post operative care.
KEYWORDS: typhoid fever, enteric perforation, peritonitis.
Article citation: Porwal, SK.,Mewara, BC.,Swarnkar, M,Gupta, S. A clinical study of enteric perforation peritonitis. J Pharm Biomed Sci. 2015 Feb;05 Suppl:S1-S4. Available at www.jpbms.info
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Copyright © 2015 Porwal, S.K.,Mewara, B.C.,Swarnkar, M.,Gupta, S. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
Ibtihal Muiz Al-Hussaini *
Affiliation:
Biology/Mycology department, College of Science, Babylon University, Iraq
The name of the department(s) and institution(s) to which the work should be attributed:
Biology/Mycology department, College of Science, Babylon University, Iraq
Address reprint requests to
* Ibtihal Muiz Al-Hussaini.
Biology/Mycology department, College of Science, Babylon University, Iraq
Article citation: Al-Hussaini IM. Detection and estimation of some active compounds of tomato plants (Solanum lycopersicum) and measure their inhibition effect on the growth of Rhizoctonia Solani. J Pharm Biomed Sci 2015 Feb; 05(02) Suppl:S19-S24. Available at www.jpbms.info
ABSTRACT: The study which includ the effect of bio-fungicides Bacillin ( consist of Bacillus cereus + active material ) with or without boron and/ or manganese used to control damping-off and root rot disease caused by Rhizoctonia solani throughout that treated of tomato seeds (Solanum lycopersicum) . Amount of Boron in leaves and root of tomato plant grown in soil free of R. solani and treated with these elements were 509.36 and 418.53 μg/g respectively. Level of Manganese which was not changed in leaves of plant from all treatments while increased in roots of Manganese treated plants (58 μg/g).
The results of experiment demonstrated that the content of phenols and alkaloids in roots of tomato plants (Solanum lycopersicum) possess antagonistic activity against R. solani in particular alkaloids which have more effectivnace on fungus than phenols. This antagonistic activity was increased with presence of Bacillin from 44.1% to 98%. Also the efficiency of alkaloids against R. solani increased by this bio-agent from 78.9% to 100%. Alkaloids compound were detected through the chromatography experiment which run at Rf = 33.5 and Rf= 7.6, also phenolic compound at Rf= 80.5 and Rf= 6.4 Both of them may play an important role in induced resistance.
KEYWORDS: Rhizoctonia solani; Tomato; Bacillin; elements; Active compounds.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Original article
Shiferaw Bekele1, Waqtola Cheneke1, Zeleke Mekonnen1,*
Affiliation:
1Department of Medical Laboratory Sciences and Pathology, Jimma University, Jimma, Ethiopia
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Medical Laboratory Sciences and Pathology, Jimma University, Jimma, Ethiopia
Address reprint requests to
* Zeleke Mekonnen
College of Health Sciences, Jimma University, P.O .box:
378, Jimma, Ethiopia
Article citation: Bekele S, Cheneke W, Mekonnen Z.Assessment of liver function tests and associated risk factors among diabetic patients attending diabetes clinic of Jimma university specialized hospital, Jimma, Ethiopia.J Pharm Biomed Sci. 2015 Feb; 05(02) Suppl:S25-S33. Available at www.jpbms.info
ABSTRACT:
Background: Patients having metabolic disorder like diabetes are more likely susceptible to liver diseases that cause the release of hepatic biomarkers. Determining those biomarkers will help in the early management of potential liver diseases;however, such studies are scarce in the present study areas. Thus,we aimed at assessing the Preualence of liver function tests and associated risk factors among diabetic patients Methods: An institution based crosssectional study was conducted. 376 diabetic patients who fulfilled the inclusion criteria were participated. 5ml venous blood was collected for liver function tests and anti-hepatitis C-virus antibody detection from each diabetic patient. Descriptive statistics, bivariate and multivariate logistic regression were performed using SPSS version 20 software. P-value less than 0.05 was considered as statistically significant. Result: Among 376 diabetic patients, 57.7% of them were found to have one or more abnormal liver function test results while 26.9% of them had at least two or more abnormal liver function test results. Out of 57.7% of diabetic patients, who had one or more abnormal liver function tests,23.9%, 20.5%,12.0%,10.1%, 9.3% and 3.2% of them had abnormal serum concentration for aspartate amino-transferase (AST), both alanine amino-transferase (ALT) and alkaline phosphatase (ALP), total bilirubin, albumin, direct bilirubin and gamma glutamyl-transferase (GGT),respectively. Out of 376 diabetic patients, 1.6% of them were found to be positive for HCV. Out of 1.6% diabetic patients who were positive for HCV, 16.0% of them had abnormal serum concentrations for AST, ALT and ALP.Elevated ALT test result had statistically significant association (p <0.05) with increasing body mass index Conclusion and recommendation:High preualence of one or more abnormal liver function test results was indicated among diabetic patients. Assessment of liver function tests and associated factors among diabetic patients during early onset of diabetes and then follow up is necessary to control and properly manage liver diseases. Health education about the potential risk of liver diseases and way of prevention shall be provided to diabetic patients as well.
KEYWORDS: Diabetes mellitus, liver function tests, alanine amino-transferases, aspartate aminotransferases, bilirubin.
AUTHOR CONTRIBUTIONS
Shiferaw Bekele, Zeleke Mekonnen and Waqtola Cheneke were involved in conception and design, and acquisition of data. Shiferaw Bekele took the lead in data generation, analysis and drafting the manuscript. Zeleke Mekonnen
and Waqtola Cheneke revised the draft manuscript critically for important intellectual content. All authors were involved in analysis and interpretation of the data, as well as final approval of the version to be published.
Source of funding: Jimma University, contract No. RPGD451/2014.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work.
There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Bekele S, Cheneke W, Mekonnen Z. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.