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Name | Validated RP - HPLC Method for the Simultaneous Determination of Amlodipine Besylate, Valsartan and Hydrochlorothiazide in Bulk and in Pharmaceutical Formulation. |
Description | Original research article:- *Jothieswari. D1, 2, Anandakumar. K2, Vijaya Santhi. D3, Vijayakumar. B4, Priya. D4, Stephen Rathinaraj. B5 1.Department of Pharmaceutical Analysis, Sri Venkateswara College of Pharmacy, Chittoor, Andhra Pradesh, India. 2.Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur, Tamilnadu, India. 3.Department of Pharmaceutical Analysis, M.A.M College of Pharmacy, Kesanupalli, Guntur, Andhra Pradesh, India. 4.Department of Pharmaceutical Chemistry, Sri Venkateswara College of Pharmacy, Chittoor, Andhra Pradesh, India. 5.Department of Pharmaceutical Analysis, Vaagdevi College of Pharmacy, Warangal, Andhra Pradesh, India.
Abstract:- A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of amlodipine besylate, valsartan and hydrochlorothiazide in pharmaceutical formulation using RP - C18 column. The mobile phase (acetonitrile: methanol: 50 mM phosphate buffer adjusted to pH 3 with orthophosphoric acid) was pumped at a flow rate of 1.0 mL min-1 in the ratio of 20: 50: 30% v/ v and the eluents were monitored at 239 nm. Linearity was obtained in the concentration range of 0.5 – 5 g mL-1 for amlodipine besylate, 4 - 40 g mL-1 for valsartan and 1 – 10 g mL-1 for hydrochlorothiazide. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining amlodipine besylate, valsartan and hydrochlorothiazide in bulk and in pharmaceutical dosage form.
Keywords: Amlodipine besylate, valsartan, hydrochlorothiazide, RP - HPLC. |
Filename | D Jothiewari et. al..pdf |
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Created On: | 04/17/2011 00:00 |
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Last updated on | 04/26/2011 16:40 |
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