Property | Value |
Name | Validation of bleach optimization for smear microscopy in pulmonary tuberculosis in resource-constrained settings |
Description | Research article:-Microbiology Bineeta Kashyap1*,Rajat Jhamb2,Prakash Kumar Mishra3,Iqbal R Kaur4. 1MBBS MD, Assistant Professor,3MBBS,Post Graduate Student,4MBBS MD, Professor & HOD, Department of Microbiology,2MBBS MD,Department of Medicine, UCMS & GTB Hospital, New Delhi, India.
Abstract:- Background and objectives: Diagnostic options for pulmonary tuberculosis in resource-poor settings are commonly limited to smear microscopy. Direct smear microscopy is inexpensive, rapid, and highly specific in settings where tuberculosis is endemic. However, direct smear microscopy has poor sensitivity (range, 20 to 80%), particularly in HIV-coinfected patients. The Stop TB Partnership Retooling Task Force identified bleach sedimentation as one of the promising approaches to improving the sensitivity of sputum smear microscopy in high-burden countries. Hence this study was initiated to see the effect of bleach optimization on sputum samples for direct microscopic diagnosis of pulmonary tuberculosis. Method: A prospective study was done from February 2011 to March 2011 at the Department of Microbiology, U.C.M.S & G.T.B hospital, Delhi. Sputum sample received were aliquoted. Smears prepared from the first aliquots were stained with Ziehl-Neelsen (ZN) stain after petroff’s concentration and cultured on LJ medium. The second aliquots of sputum sample were treated with bleach and processed with centrifugation and sedimentation techniques. Smears were stained with ZN and viewed under direct microscopy. Result: Out of 50 samples received, 4 samples (8%) were positive for AFB by ZN staining after petroff’s concentration, bleach centrifugation and bleach sedimentation techniques. All these samples were also culture positive on LJ medium. Conclusion: Bleach treatment (centrifugation and sedimentation) of sputum correlates with but does not improve the sensitivity of smear microscopy over ZN staining after petroffs concentration for the diagnosis of TB in a high TB burden area. Further evaluation of this method as a tool for tuberculosis control with more focus on alternative approaches to optimizing smear microscopy strategies is needed.
Key words:- Sputum microscopy, pulmonary tuberculosis, bleaches optimization, sedimentation, centrifugation. |
Filename | Kashyap Bineeta et al.(21-25).pdf |
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Created On: | 11/04/2012 00:00 |
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Last updated on | 11/20/2012 15:24 |
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