Property | Value |
Name | The Effects of Oral Administration of Dehydroepiandrosterone (DHEA) on Ratios of Steroidal Hormone Metabolites in Urine of Healthy Male Volunteers |
Description | Original article:- Pharmacology and Therapeutics Frans D Suyatna MD PhD and Effi Setiawati MSc Pharm. Department of Pharmacology and Therapeutics, Medical School, University of Indonesia, Salemba 6, Jakarta 10430, Indonesia.
Abstract:- Dehydroepiandrosterone (DHEA), a precursor of steroidal hormones, has been widely available as food supplement. Consumption of it, especially in the long term, may alter endogenous hormone composition in man. In the present study we investigated the effects of 50 mg DHEA orally administered in 5 consecutive days, in 13 healthy male subjects of 20-30 years old on steroidal hormone metabolites in urine. Urine samples were collected at zero time, 1, 2, 4, 5, 7, 8, 10, 12, 14 and 24 hours on day 5 after DHEA consumption and the metabolites were measured as ratios. Our results showed that the T/EpiT ratios were 1.07 ± 1.15 at zero time, peaked at 8 hours (1.18 ± 1.21), before and 1.03 ± 1.15 at zero time, peaked at 8 hours (2.11 ± 2.53) after exogenous DHEA. The A/Etio ratios were 1.45 ± 0.54 at zero time, peaked at 12 hours (1.82 ± 0.68), before and 0.77 ± 0.49 at zero time, peaked at 4 hours (1.51 ± 0.67) after exogenous DHEA. The ratios of glucuronate metabolites of 5α-androstane-3α, 17β-diol/5β-androstane-3α, 17β-diol(5α-diol/5β-diol) were 0.56 ± 0.29 at zero time, peaked at 8 hours (0.68 ± 0.40), before and 0.53 ± 0.38 at zero time, peaked at 8 hours (0.63 ±0.53), after exogenous DHEA. The DHEAS/DHEA glucuronate ratios were 20.88 ± 18.82 at zero time, peaked at 4 hours (25.60 ± 25.17), before and 67.76 ± 46.65 at zero time, peaked at 8 hours (158.03 ± 95.63), after exogenous DHEA. The DHEA levels were 30.1 ± 12.9 ng/ml at zero time and slightly fluctuated with the lowest value at 8 hours (18.99 ± 9.9) ng/ml, before and 39.6 ± 27.5 ng/ml at zero time and peaked at 4 hours ( 140.4 ± 76.4) ng/ml, after exogenous DHEA. The dehydroepiandrosterone sulfate (DHEAS) levels were 439.2±378.4 ng/ml at zero time, fluctuated with the lowest value at 8 hours (213.3 ±174.4) ng/ml, before and 1716.9 ± 1039.8 ng/ml at zero time and peaked at 8 hours (8762.8 ± 6361.0) ng/ml after exogenous DHEA. These data suggest that consumption of exogenous DHEA alters steroidal hormones composition in the body and that voluntary consumption of the substance could be predicted by determining T/epiT and DHEAS/DHEA glucuronate ratios in urine.
Key words:- A/Etio, 5α-diol/5β-diol, Dehydroepiandrosterone (DHEA), DHEAS/DHEA, GC/MSD, glucuronate, T/epiT. |
Filename | Suyatna, Frans D and Setiawati, Effi(67-73).pdf |
Filesize | 492.64 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 11/04/2012 00:00 |
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Last updated on | 11/22/2012 17:41 |
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